Document Detail


Cell-cell interactions: enhancement of glycosyl transferase ectoenzyme systems during Chlamydomonas gametic contact.
MedLine Citation:
PMID:  1054508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycosyl transferase ectoenzyme systems that transfer galactose, glucose, N-acetylglucosamine, N-acetylneuraminic acid, mannose, and fucose have been detected on vegetative cells and gametes of Chlamydomonas moewusii. Gametes have higher levels of activity of the transferase ectoenzyme systems than morphologically identical vegetative cells, as determined by transfer of monosaccharide onto endogenous cell surface acceptors. When (plus) and (minus) gametes are mixed, there is a significant increase in the activity of transferase ectoenzyme systems. No enhancement in activity of transferase ectoenzyme systems occurs when (plus) and (minus) vegetative cells are mixed. Flagellar membrane vesicles obtained from (plus) and (minus) gametes show high activity of transferase ectoenzyme systems per mg of protein and also demonstrate enhanced activity upon mixing. Therefore, glycosyl transferases and acceptors seem to be located on the flagellar membrane and appear to have a function particularly related to gametic cells. The mechanism of cellular adhesion or recognition proposed by Roseman (1970, Chem. Phys. Lipids 5, 270-297), involving glycosyl transferases and acceptors, is strongly suggested by our data for the mating reaction in Chlamydomonas.
Authors:
R J McLean; H B Bosmann
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  72     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1975 Jan 
Date Detail:
Created Date:  1975-06-11     Completed Date:  1975-06-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  310-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Binding Sites
Cell Adhesion*
Cell Membrane / enzymology
Chlamydomonas / cytology,  enzymology*
Fucose / metabolism
Galactose / metabolism
Glucosamine / metabolism
Glucose / metabolism
Hexosyltransferases / metabolism*
Mannose / metabolism
Neuraminic Acids / metabolism
Plant Proteins / metabolism
Chemical
Reg. No./Substance:
0/Neuraminic Acids; 0/Plant Proteins; 26566-61-0/Galactose; 31103-86-3/Mannose; 3416-24-8/Glucosamine; 3713-31-3/Fucose; 50-99-7/Glucose; EC 2.4.1.-/Hexosyltransferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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