Document Detail


Cell-cell fusion induced by the avian reovirus membrane fusion protein is regulated by protein degradation.
MedLine Citation:
PMID:  15140997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The p10 fusion-associated small transmembrane protein of avian reovirus induces extensive syncytium formation in transfected cells. Here we show that p10-induced cell-cell fusion is restricted by rapid degradation of the majority of newly synthesized p10. The small ectodomain of p10 targets the protein for degradation following p10 insertion into an early membrane compartment. Paradoxically, conservative amino acid substitutions in the p10 ectodomain hydrophobic patch that eliminate fusion activity also increase p10 stability. The small amount of p10 that escapes intracellular degradation accumulates at the cell surface in a relatively stable form, where it mediates cell-cell fusion as a late-stage event in the virus replication cycle. The unusual relationship between a nonstructural viral membrane fusion protein and the replication cycle of a nonenveloped virus has apparently contributed to the evolution of a novel mechanism for restricting the extent of virus-induced cell-cell fusion.
Authors:
Maya Shmulevitz; Jennifer Corcoran; Jayme Salsman; Roy Duncan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  78     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-13     Completed Date:  2004-06-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5996-6004     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Line
Membrane Fusion*
Molecular Sequence Data
Orthoreovirus, Avian / physiology*
Viral Fusion Proteins / chemistry,  physiology*
Virus Replication
Chemical
Reg. No./Substance:
0/Viral Fusion Proteins
Comments/Corrections

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