Document Detail


Cell-based selection provides novel molecular probes for cancer stem cells.
MedLine Citation:
PMID:  23150432     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cancer stem cells (CSC) represent a malignant subpopulation of cells in hierarchically organized tumors. They constitute a subpopulation of malignant cells within a tumor mass and possess the ability to self-renew giving rise to heterogeneous tumor cell populations with a complex set of differentiated tumor cells. CSC may be the cause of metastasis and therapeutic refractory disease. Because few markers exist to identify and isolate pure CSC, we used cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) to create DNA aptamers that can identify novel molecular targets on the surfaces of live CSC. Out of 22 putative DNA sequences, 3 bound to ~90% and 5 bound to ~15% of DU145 prostate cancer cells. The 15% of cells that were positive for the second panel of aptamers expressed high levels of E-cadherin and CD44, had high aldehyde dehydrogenase 1 activity, grew as spheroids under nonadherent culture conditions, and initiated tumors in immune-compromised mice. The discovery of the molecular targets of these aptamers could reveal novel CSC biomarkers.
Authors:
Kwame Sefah; Kyung-Mi Bae; Joseph A Phillips; Dietmar W Siemann; Zhen Su; Steve McClellan; Johannes Vieweg; Weihong Tan
Publication Detail:
Type:  Journal Article     Date:  2013-02-08
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  132     ISSN:  1097-0215     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-03-26     Completed Date:  2013-05-28     Revised Date:  2014-07-04    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2578-88     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 UICC.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aptamers, Nucleotide
Flow Cytometry
Humans
Image Processing, Computer-Assisted
Immunophenotyping
Male
Mice
Molecular Probes / diagnostic use*
Neoplastic Stem Cells / pathology*
Prostatic Neoplasms / diagnosis,  metabolism*
SELEX Aptamer Technique
Spheroids, Cellular
Tumor Cells, Cultured
Tumor Markers, Biological / metabolism*
Grant Support
ID/Acronym/Agency:
R01 GM079359/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 0/Molecular Probes; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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