Document Detail

Cell-based screening for membranal and cytoplasmatic markers using dielectric spectroscopy.
MedLine Citation:
PMID:  18433979     Owner:  NLM     Status:  MEDLINE    
Dielectric spectroscopy (DS) of living biological cells is based on the analysis of the complex dielectric permittivity of cells suspended in a physiological medium. It provides knowledge on the polarization-relaxation response of cells to external electric field as function of the excitation frequency. This response is strongly affected by both structural and molecular properties of cells and therefore, can reveal rare insights on cell physiology and behaviour. This study demonstrates the mapping potential of DS after cytoplasmatic and membranal markers for cell-based screening analysis. The effect of membrane permittivity and cytoplasm conductivity was examined using tagged MBA and MDCK cell lines respectively. Comparing the permittivity spectra of tagged and native cell lines reveals clear differences between the analyzed suspensions. In addition, differences on the matching dielectric properties of cells were obtained. Those findings support the high distinction resolution and sensitivity of DS after fine molecular and cellular changes, and hence, highlight the high potential of DS as non invasive screening tool in cell biology research.
Amit Ron; Ragini Raj Singh; Nick Fishelson; Irena Shur; Rina Socher; Dafna Benayahu; Yosi Shacham-Diamand
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Publication Detail:
Type:  Journal Article     Date:  2008-03-29
Journal Detail:
Title:  Biophysical chemistry     Volume:  135     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-09-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  59-68     Citation Subset:  IM    
Department of Electrical Engineering, Faculty of Engineering, Tel-Aviv University, Israel.
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MeSH Terms
Biological Markers*
Cell Line
Cell Membrane / physiology*
Cytoplasm / physiology*
Electric Capacitance
Electromagnetic Fields
Epithelial Cells / cytology,  physiology
Kidney / cytology
Mass Screening
Microscopy, Atomic Force
Models, Biological*
Osteoblasts / cytology,  physiology
Spectrum Analysis
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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