Document Detail

Cell-based chip for the detection of anticancer effect on HeLa cells using cyclic voltammetry.
MedLine Citation:
PMID:  18782663     Owner:  NLM     Status:  MEDLINE    
HeLa cells directly immobilized on gold-patterned silicon substrate were used to assess the biological toxicity of anticancer drugs (hydroxyurea and cyclophosphamide). Immobilization of HeLa cells was confirmed by optical microscopy, and cell growth, viability and drug-related toxicity were examined by cyclic voltammetry and potentiometric stripping analysis. The voltammetric behaviors of HeLa cells displayed a quasi-reversible pattern with the peak current exhibiting a linear relationship with cell number. The attached living cells were exposed to different concentrations of hydroxyurea and cyclophosphamide as anticancer drugs, which induced the change of cyclic voltammetry current peak. As the exposed concentration of anticancer drugs was increased, the change of current peak was increased, which indicates the decrease of cell viability. Trypan Blue dyeing was performed to confirm the results of the effect of anticancer drugs on the cell viability which was obtained from cyclic voltammetry assay. The proposed direct cell immobilization method technique can be applied to the fabrication of cell chip for diagnosis, drug detection, and on-site monitoring.
Waleed Ahmed El-Said; Cheol-Heon Yea; Hyunhee Kim; Byung-Keun Oh; Jeong-Woo Choi
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-31
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  24     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-12     Completed Date:  2009-03-09     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1259-65     Citation Subset:  IM    
Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-dong Mapo-gu, Seoul 121-742, Republic of Korea.
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MeSH Terms
Antineoplastic Agents / administration & dosage*
Biological Assay / instrumentation*
Biosensing Techniques / instrumentation*,  methods
Cell Survival / drug effects*
Drug Evaluation, Preclinical / instrumentation*,  methods
Electrochemistry / instrumentation*
Equipment Design
Equipment Failure Analysis
Hela Cells
Microfluidic Analytical Techniques / instrumentation*
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Antineoplastic Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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