| Cell-based assays in practice: cell markers from autofluorescent proteins of the GFP-family. | |
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MedLine Citation:
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PMID: 18795879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The more recently discovered anthozoan fluorescent proteins (FPs) and the classic Aequorea victoria Green Fluorescent Protein (avGFP) as well as their derivatives have become versatile tools as live cell markers in fluorescence microscopy. In this review, we show the use of these FPs in drug discovery assays. Assay examples are given for the application of FPs in multiplexed imaging, as photosensitizers, as fluorescent timers, as pulse-chase labels and for robotically integrated compound testing. The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy combined with image analysis to pharmaceutical high throughput drug discovery assays, generally referred to as High Content Screening (HCS). |
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Authors:
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Michael Wolff; Simone Kredel; Jörg Wiedenmann; G Ulrich Nienhaus; Ralf Heilker |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Combinatorial chemistry & high throughput screening Volume: 11 ISSN: 1386-2073 ISO Abbreviation: Comb. Chem. High Throughput Screen. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-17 Completed Date: 2008-10-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810948 Medline TA: Comb Chem High Throughput Screen Country: Netherlands |
Other Details:
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Languages: eng Pagination: 602-9 Citation Subset: IM |
Affiliation:
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Department of Lead Discovery, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, Biberach, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / chemistry* CHO Cells Cells, Cultured Cricetinae Cricetulus Green Fluorescent Proteins / chemistry* Microscopy, Fluorescence* Reactive Oxygen Species / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Reactive Oxygen Species; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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