Document Detail

Cell-based assays in practice: cell markers from autofluorescent proteins of the GFP-family.
MedLine Citation:
PMID:  18795879     Owner:  NLM     Status:  MEDLINE    
The more recently discovered anthozoan fluorescent proteins (FPs) and the classic Aequorea victoria Green Fluorescent Protein (avGFP) as well as their derivatives have become versatile tools as live cell markers in fluorescence microscopy. In this review, we show the use of these FPs in drug discovery assays. Assay examples are given for the application of FPs in multiplexed imaging, as photosensitizers, as fluorescent timers, as pulse-chase labels and for robotically integrated compound testing. The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy combined with image analysis to pharmaceutical high throughput drug discovery assays, generally referred to as High Content Screening (HCS).
Michael Wolff; Simone Kredel; Jörg Wiedenmann; G Ulrich Nienhaus; Ralf Heilker
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Combinatorial chemistry & high throughput screening     Volume:  11     ISSN:  1386-2073     ISO Abbreviation:  Comb. Chem. High Throughput Screen.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-17     Completed Date:  2008-10-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810948     Medline TA:  Comb Chem High Throughput Screen     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  602-9     Citation Subset:  IM    
Department of Lead Discovery, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, Biberach, Germany.
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MeSH Terms
Biological Markers / chemistry*
CHO Cells
Cells, Cultured
Green Fluorescent Proteins / chemistry*
Microscopy, Fluorescence*
Reactive Oxygen Species / chemistry
Reg. No./Substance:
0/Biological Markers; 0/Reactive Oxygen Species; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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