Document Detail


Cell-associated nonesterified fatty acid levels and their alteration during lipolysis in the isolated mouse adipose cell.
MedLine Citation:
PMID:  4355095     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and flexible method has been developed for measuring cell-associated, probably intracellular, nonesterified fatty acids (CAFA) in isolated mouse adipose cells. A variety of lipolytic agents as well as various concentrations of epinephrine elevate CAFA levels in rough proportion to their stimulation of glycerol and fatty acid release. Insulin reduces epinephrine-elevated CAFA levels. A detailed, quantitative study of the relationship among lipolytic activity, CAFA levels, and the extracellular molar ratio of fatty acids to albumin has been carried out. Epinephrine-elevated CAFA levels rise linearly with, while epinephrine-stimulated lipolytic activity is independent of, fatty acid to albumin ratios below 2-3. As the ratio increases from 3 to 5, CAFA levels continue to increase, whereas lipolytic activity decreases. Above ratios of 5, fatty acid release almost completely ceases; CAFA levels increase dramatically with residual glycerol release. A temperature-dependent efflux of epinephrine-elevated CAFA can be elicited through blockade of stimulated lipolysis with propranolol, but only in the presence of extracellular fatty acid to albumin ratios below 3. These observations suggest that during stimulated lipolysis, a fatty acid gradient exists between the cell and extracellular serum albumin and that CAFA represent the intracellular component of this gradient. In addition, these observations support the concept that intracellular fatty acids play a role in the feedback regulation of adipose cell function as extracellular fatty acids accumulate during the lipolytic response.
Authors:
S W Cushman; J J Heindel; B Jeanrenaud
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  14     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1973 Nov 
Date Detail:
Created Date:  1973-12-12     Completed Date:  1973-12-12     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  632-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / cytology,  metabolism*
Adrenocorticotropic Hormone / pharmacology
Animals
Cyclic AMP / pharmacology
Epididymis
Epinephrine / pharmacology
Fatty Acids, Nonesterified / analysis,  metabolism*
Feedback
Glucose
Glutaral
Glycerol / analysis
Insulin / pharmacology
Lipid Mobilization / drug effects*
Male
Mice
Oleic Acids / pharmacology
Propranolol / pharmacology
Pyruvates
Serum Albumin
Chemical
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Oleic Acids; 0/Pyruvates; 0/Serum Albumin; 11061-68-0/Insulin; 111-30-8/Glutaral; 50-99-7/Glucose; 51-43-4/Epinephrine; 525-66-6/Propranolol; 56-81-5/Glycerol; 60-92-4/Cyclic AMP; 9002-60-2/Adrenocorticotropic Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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