Document Detail


Cell analysis using a multiple internal reflection photonic lab-on-a-chip.
MedLine Citation:
PMID:  21959243     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Here we present a protocol for analyzing cell cultures using a photonic lab-on-a-chip (PhLoC). By using a broadband light source and a spectrometer, the spectrum of a given cell culture with an arbitrary population is acquired. The PhLoC can work in three different regimes: light scattering (using label-free cells), light scattering plus absorption (using stained cells) and, by subtraction of the two former regimes, absorption (without the scattering band). The acquisition time of the PhLoC is ∼30 ms. Hence, it can be used for rapid cell counting, dead/live ratio estimation or multiparametric measurements through the use of different dyes. The PhLoC, including microlenses, micromirrors and microfluidics, is simply fabricated in a single-mask process (by soft lithographic methods) using low-cost materials. Because of its low cost it can easily be implemented for point-of-care applications. From raw substrates to final results, this protocol can be completed in 29 h.
Authors:
Jordi Vila-Planas; Elisabet Fernández-Rosas; Bergoi Ibarlucea; Stefanie Demming; Carme Nogués; Jose A Plaza; Carlos Domínguez; Stephanus Büttgenbach; Andreu Llobera
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Publication Detail:
Type:  Journal Article     Date:  2011-09-29
Journal Detail:
Title:  Nature protocols     Volume:  6     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1642-55     Citation Subset:  IM    
Affiliation:
Centre Nacional de Microelectrònica (IMB-CNM, CSIC), Barcelona, Spain.
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