Document Detail


Cell death/proliferation and alterations in glial morphology contribute to changes in diffusivity in the rat hippocampus after hypoxia-ischemia.
MedLine Citation:
PMID:  20877389     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To understand the structural alterations that underlie early and late changes in hippocampal diffusivity after hypoxia/ischemia (H/I), the changes in apparent diffusion coefficient of water (ADC(W)) were studied in 8-week-old rats after H/I using diffusion-weighted magnetic resonance imaging (DW-MRI). In the hippocampal CA1 region, ADC(W) analyses were performed during 6 months of reperfusion and compared with alterations in cell number/cell-type composition, glial morphology, and extracellular space (ECS) diffusion parameters obtained by the real-time iontophoretic method. In the early phases of reperfusion (1 to 3 days) neuronal cell death, glial proliferation, and developing gliosis were accompanied by an ADC(W) decrease and tortuosity increase. Interestingly, ECS volume fraction was decreased only first day after H/I. In the late phases of reperfusion (starting 1 month after H/I), when the CA1 region consisted mainly of microglia, astrocytes, and NG2-glia with markedly altered morphology, ADC(W), ECS volume fraction and tortuosity were increased. Three-dimensional confocal morphometry revealed enlarged astrocytes and shrunken NG2-glia, and in both the contribution of cell soma/processes to total cell volume was markedly increased/decreased. In summary, the ADC(W) increase in the CA1 region underlain by altered cellular composition and glial morphology suggests that considerable changes in extracellular signal transmission might occur in the late phases of reperfusion after H/I.
Authors:
Miroslava Anderova; Ivan Vorisek; Helena Pivonkova; Jana Benesova; Lydia Vargova; Michal Cicanic; Alexandr Chvatal; Eva Sykova
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-29
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  31     ISSN:  1559-7016     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-01     Completed Date:  2011-04-27     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  894-907     Citation Subset:  IM    
Affiliation:
Institute of Experimental Medicine, Department of Cellular Neurophysiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. anderova@biomed.cas.cz
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MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia / complications,  pathology*,  physiopathology
Astrocytes / pathology
Body Water / metabolism*
Brain Ischemia / complications,  pathology*,  physiopathology*
CA1 Region, Hippocampal / pathology*,  physiopathology
Cell Count
Cell Death
Cell Proliferation*
Diffusion
Diffusion Magnetic Resonance Imaging
Extracellular Space / metabolism
Gliosis / etiology,  pathology
Imaging, Three-Dimensional
Immunohistochemistry
Male
Microscopy, Confocal
Neuroglia / pathology*
Rats
Rats, Wistar
Reperfusion
Time Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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