Document Detail


Cell adhesion. The minimal cadherin-catenin complex binds to actin filaments under force.
MedLine Citation:
PMID:  25359979     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Linkage between the adherens junction (AJ) and the actin cytoskeleton is required for tissue development and homeostasis. In vivo findings indicated that the AJ proteins E-cadherin, β-catenin, and the filamentous (F)-actin binding protein αE-catenin form a minimal cadherin-catenin complex that binds directly to F-actin. Biochemical studies challenged this model because the purified cadherin-catenin complex does not bind F-actin in solution. Here, we reconciled this difference. Using an optical trap-based assay, we showed that the minimal cadherin-catenin complex formed stable bonds with an actin filament under force. Bond dissociation kinetics can be explained by a catch-bond model in which force shifts the bond from a weakly to a strongly bound state. These results may explain how the cadherin-catenin complex transduces mechanical forces at cell-cell junctions.
Authors:
Craig D Buckley; Jiongyi Tan; Karen L Anderson; Dorit Hanein; Niels Volkmann; William I Weis; W James Nelson; Alexander R Dunn
Related Documents :
15582779 - The relative role of the t-domain and flanking sequences for developmental control and ...
15595839 - Engineering the loop region of a homeodomain-leucine zipper protein promotes efficient ...
9257679 - Conservative val47 residue of pou homeodomain: role in dna recognition.
7753559 - Differential transcriptional regulation of c-myc promoter through the same dna binding ...
9556579 - Site-directed mutations in the vnd/nk-2 homeodomain. basis of variations in structure a...
19922439 - Activation of krox20 gene expression by sox10 in myelinating schwann cells.
3295229 - A thermodynamic approach to hormone-receptor interaction; application to insulin bindin...
11854289 - Hybrid tetramers of porcine liver fructose-1,6-bisphosphatase reveal multiple pathways ...
19296449 - Functional screening of adrenergic receptors by measuring intracellular calcium using t...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  346     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1254211     Citation Subset:  IM    
Copyright Information:
Copyright © 2014, American Association for the Advancement of Science.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
1DP2OD007078/OD/NIH HHS; GM35527/GM/NIGMS NIH HHS; GM56169/GM/NIGMS NIH HHS; P01GM098412/GM/NIGMS NIH HHS; U01GM094663/GM/NIGMS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Plant genetics. A Y-chromosome-encoded small RNA acts as a sex determinant in persimmons.
Next Document:  Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coile...