Document Detail


Cell adaptation to solvent, substrate and product: a successful strategy to overcome product inhibition in a bioconversion system.
MedLine Citation:
PMID:  15841371     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carvone has previously been found to highly inhibit its own production at concentrations above 50 mM during conversion of a diastereomeric mixture of (-)-carveol by whole cells of Rhodococcus erythropolis. Adaptation of the cells to the presence of increasing concentrations of carveol and carvone in n-dodecane prior to biotransformation proved successful in overcoming carvone inhibition. By adapting R. erythropolis cells for 197 h, an 8.3-fold increase in carvone production rate compared to non-adapted cells was achieved in an air-driven column reactor. After an incubation period of 268 h, a final carvone concentration of 1.03 M could be attained, together with high productivity [0.19 mg carvone h(-1) (ml organic phase)(-1)] and high yield (0.96 g carvone g carveol(-1)).
Authors:
Carla C C R de Carvalho; Alessandro Poretti; M Manuela R da Fonseca
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-15
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  69     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-28     Completed Date:  2006-02-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  268-75     Citation Subset:  IM    
Affiliation:
Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. ccarvalho@ist.utl.pt
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / physiology*
Bacterial Physiological Phenomena*
Bioreactors*
Cell Culture Techniques / methods*
Cell Survival / drug effects
Dose-Response Relationship, Drug
Monoterpenes / metabolism,  pharmacology*
Rhodococcus / enzymology,  metabolism*
Solvents / adverse effects,  chemistry
Substrate Specificity
Water / chemistry
Chemical
Reg. No./Substance:
0/Monoterpenes; 0/Solvents; 7732-18-5/Water; 99-49-0/carvone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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