| Cell adaptation to solvent, substrate and product: a successful strategy to overcome product inhibition in a bioconversion system. | |
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MedLine Citation:
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PMID: 15841371 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Carvone has previously been found to highly inhibit its own production at concentrations above 50 mM during conversion of a diastereomeric mixture of (-)-carveol by whole cells of Rhodococcus erythropolis. Adaptation of the cells to the presence of increasing concentrations of carveol and carvone in n-dodecane prior to biotransformation proved successful in overcoming carvone inhibition. By adapting R. erythropolis cells for 197 h, an 8.3-fold increase in carvone production rate compared to non-adapted cells was achieved in an air-driven column reactor. After an incubation period of 268 h, a final carvone concentration of 1.03 M could be attained, together with high productivity [0.19 mg carvone h(-1) (ml organic phase)(-1)] and high yield (0.96 g carvone g carveol(-1)). |
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Authors:
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Carla C C R de Carvalho; Alessandro Poretti; M Manuela R da Fonseca |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-11-15 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 69 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-12-28 Completed Date: 2006-02-09 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 268-75 Citation Subset: IM |
Affiliation:
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Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. ccarvalho@ist.utl.pt |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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physiology* Bacterial Physiological Phenomena* Bioreactors* Cell Culture Techniques / methods* Cell Survival / drug effects Dose-Response Relationship, Drug Monoterpenes / metabolism, pharmacology* Rhodococcus / enzymology, metabolism* Solvents / adverse effects, chemistry Substrate Specificity Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Monoterpenes; 0/Solvents; 7732-18-5/Water; 99-49-0/carvone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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