Document Detail


Cell Volume Changes During Apoptosis Monitored in Real Time Using Digital Holographic Microscopy.
MedLine Citation:
PMID:  22465356     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cellular volume changes play important roles in many processes associated with the normal cell activity, as well as various diseases. Consequently, there is a considerable need to accurately measure volumes of both individual cells and cell populations as a function of time. In this study, we have monitored cell volume changes in real time during apoptosis using digital holographic microscopy. Cell volume changes were deduced from the measured phase change of light transmitted through cells. Our digital holographic experiments showed that after exposure to 1 μM staurosporine for four hours, the volumes of KB cells were reduced by ∼50-60%, which is consistent with previous results obtained using electronic cell sizing and atomic force microscopy. In comparison with other techniques, digital holographic microscopy is advantageous because it employs noninvasive detection, has high time resolution, real time measurement capability, and the ability to simultaneously investigate time-dependent volume changes of both individual cells and cell populations.
Authors:
Alexander Khmaladze; Rebecca L Matz; Tamir Epstein; Joshua Jasensky; Mark M Banaszak Holl; Zhan Chen
Related Documents :
3225736 - Quantitative cytological analysis of functional changes in adrenomedullary chromaffin c...
19500566 - Four distinct phases of basket/stellate cell migration after entering their final desti...
17930726 - Multistability and clustering in a population of synthetic genetic oscillators via phas...
188736 - Proliferation kinetics and cyclic amp in human leukaemic cells: their relevance to ther...
8781326 - Bile ductule formation in fetal, neonatal, and infant livers compared with extrahepatic...
1299366 - Pattern formation during gastrulation in the sea urchin embryo.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-28
Journal Detail:
Title:  Journal of structural biology     Volume:  -     ISSN:  1095-8657     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-4-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9011206     Medline TA:  J Struct Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Physician experiences transitioning between an older versus newer electronic health record for elect...
Next Document:  Lipidomic analysis of the liver identifies changes of major and minor lipid species in adiponectin d...