Document Detail

Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy.
MedLine Citation:
PMID:  23150368     Owner:  NLM     Status:  MEDLINE    
Identification and visualization of specific cells and cellular structures in the retina are fundamental for understanding the visual process, retinal development, disease progression, and therapeutic intervention. The increased usage of transgenic and naturally occurring mutant mice has further emphasized the need for retinal cell-specific imaging. Immunofluorescence microscopy of retinal cryosections and whole mount tissue labeled with cell-specific markers has emerged as the method of choice for identifying specific cell populations and mapping their distribution within the retina. In most cases indirect labeling methods are employed in which lightly fixed retinal samples are first labeled with a primary antibody targeted against a cell-specific protein of interest and then labeled with a fluorescent dye-tagged secondary antibody that recognizes the primary antibody. The localization and relative abundance of the protein can readily be imaged under a conventional fluorescent or confocal scanning microscope. Immunofluorescence labeling can be adapted for imaging more than one protein antigen through the use of multiple antibodies and different, nonoverlapping fluorescent dyes. A number of well-characterized immunochemical markers are now available for detecting photoreceptors, bipolar cells, amacrine cells, horizontal cells, Müller cells, and retinal pigment epithelial cells in the retina of mice, and other mammals.
Christiana L Cheng; Hidayat Djajadi; Robert S Molday
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  935     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-17     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  185-99     Citation Subset:  IM    
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MeSH Terms
Cryopreservation / methods
Cryoultramicrotomy / methods
Dissection / methods
Fluorescent Antibody Technique / methods*
Microscopy, Fluorescence / methods*
Retina / cytology*,  ultrastructure
Staining and Labeling / methods
Tissue Fixation / methods
Grant Support
RMF-92101//Canadian Institutes of Health Research

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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