| Cell ingression and apical shape oscillations during dorsal closure in Drosophila. | |
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MedLine Citation:
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PMID: 22404919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Programmed patterns of gene expression, cell-cell signaling, and cellular forces cause morphogenic movements during dorsal closure. We investigated the apical cell-shape changes that characterize amnioserosa cells during dorsal closure in Drosophila embryos with in vivo imaging of green-fluorescent-protein-labeled DE-cadherin. Time-lapsed, confocal images were assessed with a novel segmentation algorithm, Fourier analysis, and kinematic and dynamical modeling. We found two generic processes, reversible oscillations in apical cross-sectional area and cell ingression characterized by persistent loss of apical area. We quantified a time-dependent, spatially-averaged sum of intracellular and intercellular forces acting on each cell's apical belt of DE-cadherin. We observed that a substantial fraction of amnioserosa cells ingress near the leading edges of lateral epidermis, consistent with the view that ingression can be regulated by leading-edge cells. This is in addition to previously observed ingression processes associated with zipping and apoptosis. Although there is cell-to-cell variability in the maximum rate for decreasing apical area (0.3-9.5 μm(2)/min), the rate for completing ingression is remarkably constant (0.83 cells/min, r(2) > 0.99). We propose that this constant ingression rate contributes to the spatiotemporal regularity of mechanical stress exerted by the amnioserosa on each leading edge during closure. |
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Authors:
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Adam Sokolow; Yusuke Toyama; Daniel P Kiehart; Glenn S Edwards |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-03-06 |
Journal Detail:
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Title: Biophysical journal Volume: 102 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-03-12 Completed Date: 2012-07-05 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 969-79 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Physics Department, Duke University, Durham, North Carolina, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cadherins / metabolism Cell Polarity Cell Shape* Drosophila melanogaster / cytology, embryology* Embryo, Nonmammalian / cytology*, metabolism Embryonic Development* Models, Biological* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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GM 33830/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cadherins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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