Document Detail

Cell fusion-induced activation of interferon-stimulated genes is not required for restriction of a herpes simplex virus VP16/ICP0 mutant in heterokarya formed between permissive and restrictive cells.
MedLine Citation:
PMID:  19535444     Owner:  NLM     Status:  MEDLINE    
Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction.
Meaghan H Hancock; Karen L Mossman; James R Smiley
Related Documents :
19016434 - Expression and purification of zebra fusion proteins and applications for the delivery ...
16328984 - Controlled growth of chinese hamster ovary cells and high expression of antibody-il-2 f...
3890734 - Parental age and the life-span of zygotes of saccharomyces cerevisiae.
11952834 - Tropomyosin is required for the cell fusion process during conjugation in fission yeast.
17065324 - Modeling dual pathways for the metazoan spindle assembly checkpoint.
17999784 - Murine tracheal and nasal septal epithelium for air-liquid interface cultures: a compar...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-17
Journal Detail:
Title:  Journal of virology     Volume:  83     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-08-31     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8976-9     Citation Subset:  IM    
Alberta Institute for Viral Immunology, Department of Medical Microbiology & Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Fusion*
Cell Line, Tumor
Cercopithecus aethiops
Herpes Simplex Virus Protein Vmw65 / deficiency*
Immediate-Early Proteins / deficiency*
Interferons / biosynthesis*
Simplexvirus / immunology*,  physiology*
Ubiquitin-Protein Ligases / deficiency*
Vero Cells
Virus Replication*
Reg. No./Substance:
0/Herpes Simplex Virus Protein Vmw65; 0/Immediate-Early Proteins; 9008-11-1/Interferons; EC Ligases; EC protein, Human herpesvirus 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The infectious molecular clone and pseudotyped virus models of human immunodeficiency virus type 1 e...
Next Document:  Epigenetic modulation of gene expression from quiescent herpes simplex virus genomes.