Document Detail


Cell fusion-induced activation of interferon-stimulated genes is not required for restriction of a herpes simplex virus VP16/ICP0 mutant in heterokarya formed between permissive and restrictive cells.
MedLine Citation:
PMID:  19535444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction.
Authors:
Meaghan H Hancock; Karen L Mossman; James R Smiley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-17
Journal Detail:
Title:  Journal of virology     Volume:  83     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-08-31     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8976-9     Citation Subset:  IM    
Affiliation:
Alberta Institute for Viral Immunology, Department of Medical Microbiology & Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion*
Cell Line, Tumor
Cercopithecus aethiops
Herpes Simplex Virus Protein Vmw65 / deficiency*
Humans
Immediate-Early Proteins / deficiency*
Interferons / biosynthesis*
Simplexvirus / immunology*,  physiology*
Ubiquitin-Protein Ligases / deficiency*
Vero Cells
Virus Replication*
Chemical
Reg. No./Substance:
0/Herpes Simplex Virus Protein Vmw65; 0/Immediate-Early Proteins; 9008-11-1/Interferons; EC 6.3.2.19/Ubiquitin-Protein Ligases; EC 6.3.2.19/Vmw110 protein, Human herpesvirus 1
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