| Cell fusion-induced activation of interferon-stimulated genes is not required for restriction of a herpes simplex virus VP16/ICP0 mutant in heterokarya formed between permissive and restrictive cells. | |
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MedLine Citation:
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PMID: 19535444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction. |
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Authors:
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Meaghan H Hancock; Karen L Mossman; James R Smiley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-17 |
Journal Detail:
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Title: Journal of virology Volume: 83 ISSN: 1098-5514 ISO Abbreviation: J. Virol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-11 Completed Date: 2009-08-31 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 8976-9 Citation Subset: IM |
Affiliation:
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Alberta Institute for Viral Immunology, Department of Medical Microbiology & Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion* Cell Line, Tumor Cercopithecus aethiops Herpes Simplex Virus Protein Vmw65 / deficiency* Humans Immediate-Early Proteins / deficiency* Interferons / biosynthesis* Simplexvirus / immunology*, physiology* Ubiquitin-Protein Ligases / deficiency* Vero Cells Virus Replication* |
| Chemical | |
Reg. No./Substance:
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0/Herpes Simplex Virus Protein Vmw65; 0/Immediate-Early Proteins; 9008-11-1/Interferons; EC 6.3.2.19/Ubiquitin-Protein Ligases; EC 6.3.2.19/Vmw110 protein, Human herpesvirus 1 |
| Comments/Corrections | |
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