Document Detail

Cell-Free Synthesis of Pectin (Identification and Partial Characterization of Polygalacturonate 4-[alpha]-Galacturonosyltransferase and Its Products from Membrane Preparations of Tobacco Cell-Suspension Cultures).
MedLine Citation:
PMID:  12228586     Owner:  NLM     Status:  Publisher    
Polygalacturonate 4-[alpha]-galacturonosyltransferase (EC activity has been identified in microsomal membranes isolated from tobacco (Nicotiana tabacum L. cv Samsun) cell-suspension cultures. Incubation of UDP-[14C]galacturonic acid with tobacco membranes results in a time-dependent incorporation of [14C]galacturonic acid into a chloroform-methanol-precipitable and 65% ethanol-insoluble product. The optimal synthesis of product occurs at a pH of 7.8, 25 to 30[deg]C, an apparent Km for UDP-D-galacturonic acid of approximately 8.9 [mu]M, and a Vmax of approximately 150 pmol min-1 mg-1 protein. The product was characterized by scintillation counting, thin-layer chromatography, high-performance anion-exchange chromatography, and gel-filtration chromatography in combination with enzymatic and chemical treatments. The intact product has a molecular mass of approximately 105,000 D based on dextran molecular standards. The product was treated with base to hydrolyze ester linkages (e.g. methyl esters), digested with a homogeneous endopolygalacturonase (EPGase), or base and EPGase treated. Base and EPGase treatment results in cleavage of 34 to 89% of 14C-labeled product into components that co-chromatograph with mono-, di-, and trigalacturonic acid, indicating that a large portion of product contains contiguous 1,4-linked [alpha]-D-galactosyluronic acid residues. Optimal EPGase fragmentation of the product requires base treatment prior to enzymatic digestion, suggesting that 45 to 67% of the galacturonic acid residues in the synthesized homogalacturonan are esterified. At least 40% of the base-sensitive linkages were shown to be methyl esters by comparing the sensitivity of base-treated and pectin methylesterase-treated products to fragmentation by EPGase.
R. L. Doong; K. Liljebjelke; G. Fralish; A. Kumar; D. Mohnen
Related Documents :
22629206 - The influence of season on the gonad index and biochemical composition of the sea urchi...
3084256 - The increase by spermidine of fidelity of protamine synthesis in a wheat-germ cell-free...
18967966 - Titration of strong and weak acids by sequential injection analysis technique.
20367666 - Lactobionic acid in a natural alkylpolyglucoside-based vehicle: assessing safety and ef...
3512256 - Retinoic acid enhances cell responses to epidermal growth factor in mouse mammary gland...
3707416 - Ascorbic acid levels in the aqueous humor of nocturnal and diurnal mammals.
Publication Detail:
Journal Detail:
Title:  Plant physiology     Volume:  109     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  2002-Sep-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  141-152     Citation Subset:  -    
Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, 220 Riverbend Road, Athens, Georgia 30602-4712.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A Cytochrome P-450 Monooxygenase Catalyzes the First Step in the Conversion of Tabersonine to Vindol...
Next Document:  Urease Is Not Essential for Ureide Degradation in Soybean.