Document Detail


Cell-ECM traction force modulates endogenous tension at cell-cell contacts.
MedLine Citation:
PMID:  21383129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells in tissues are mechanically coupled both to the ECM and neighboring cells, but the coordination and interdependency of forces sustained at cell-ECM and cell-cell adhesions are unknown. In this paper, we demonstrate that the endogenous force sustained at the cell-cell contact between a pair of epithelial cells is approximately 100 nN, directed perpendicular to the cell-cell interface and concentrated at the contact edges. This force is stably maintained over time despite significant fluctuations in cell-cell contact length and cell morphology. A direct relationship between the total cellular traction force on the ECM and the endogenous cell-cell force exists, indicating that the cell-cell tension is a constant fraction of the cell-ECM traction. Thus, modulation of ECM properties that impact cell-ECM traction alters cell-cell tension. Finally, we show in a minimal model of a tissue that all cells experience similar forces from the surrounding microenvironment, despite differences in the extent of cell-ECM and cell-cell adhesion. This interdependence of cell-cell and cell-ECM forces has significant implications for the maintenance of the mechanical integrity of tissues, mechanotransduction, and tumor mechanobiology.
Authors:
Venkat Maruthamuthu; Benedikt Sabass; Ulrich S Schwarz; Margaret L Gardel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-07
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-23     Completed Date:  2011-05-26     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4708-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion / physiology
Cell Communication / physiology*
Cell Line
Dogs
Epithelial Cells / cytology,  physiology*
Extracellular Matrix / physiology*
Mechanotransduction, Cellular / physiology*
Neoplasms / metabolism,  pathology,  physiopathology
Grant Support
ID/Acronym/Agency:
DP1 OD003354/OD/NIH HHS; DP1 OD003354-01/OD/NIH HHS; DP1 OD003354-02/OD/NIH HHS; DP1 OD003354-03/OD/NIH HHS; DP1 OD003354-04/OD/NIH HHS; DP10D00354/DP/NCCDPHP CDC HHS
Comments/Corrections

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