Document Detail

Cell-directed integration into three-dimensional lipid-silica nanostructured matrices.
MedLine Citation:
PMID:  20849120     Owner:  NLM     Status:  MEDLINE    
We report a unique approach in which living cells direct their integration into 3D solid-state nanostructures. Yeast cells deposited on a weakly condensed lipid/silica thin film mesophase actively reconstruct the surface to create a fully 3D bio/nano interface, composed of localized lipid bilayers enveloped by a lipid/silica mesophase, through a self-catalyzed silica condensation process. Remarkably, this integration process selects exclusively for living cells over the corresponding apoptotic cells (those undergoing programmed cell death), via the development of a pH gradient, which catalyzes silica deposition and the formation of a coherent interface between the cell and surrounding silica matrix. Added long-chain lipids or auxiliary nanocomponents are localized within the pH gradient, allowing the development of complex active and accessible bio/nano interfaces not achievable by other synthetic methods. Overall, this approach provides the first demonstration of active cell-directed integration into a nominally solid-state three-dimensional architecture. It promises a new means to integrate "bio" with "nano" into platforms useful to study and manipulate cellular behavior at the individual cell level and to interface living organisms with electronics, photonics, and fluidics.
Jason C Harper; Constantine Y Khripin; Constantine Y Khirpin; Eric C Carnes; Carlee E Ashley; DeAnna M Lopez; Travis Savage; Howland D T Jones; Ryan W Davis; Dominique E Nunez; Lina M Brinker; Bryan Kaehr; Susan M Brozik; C Jeffrey Brinker
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  ACS nano     Volume:  4     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-26     Completed Date:  2011-02-02     Revised Date:  2011-05-12    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5539-50     Citation Subset:  IM    
Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131, USA.
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MeSH Terms
Biocompatible Materials
Biosensing Techniques
Hydrogen-Ion Concentration
Lipid Bilayers
Lipids / chemistry*
Materials Testing
Microscopy, Confocal / methods
Microscopy, Electron, Scanning / methods*
Microscopy, Fluorescence / methods
Nanostructures / chemistry*
Nanotechnology / methods
Saccharomyces cerevisiae / metabolism
Scattering, Radiation
Silicon Dioxide / chemistry*
Surface Properties
Reg. No./Substance:
0/Biocompatible Materials; 0/Lipid Bilayers; 0/Lipids; 7631-86-9/Silicon Dioxide
Erratum In:
ACS Nano. 2011 Mar 22;5(3):2412
Note: Khirpin, Constantine Y [corrected to Khripin, Constantine Y]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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