| Cell Derived Liposomes Expressing CCR5 as A New Targeted Drug-Delivery System for HIV Infected Cells. | |
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MedLine Citation:
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PMID: 21362450 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Anti-retroviral-therapies against HIV/AIDS focus on inhibiting viral growth and may slow AIDS progression, but not cure the disease. Here we describe an approach to treat HIV as a cellular pathology by targeting cell derived liposomes against HIV-infected cells. Cell-derived-liposomes were prepared from the cytoplasmatic membranes of cells expressing CCR5, the human receptor for gp120, that is found on the surface of virions and HIV-infected cells. The specific targeting and cytotoxicity of the cell-derived liposomes towards gp120-expressing cells was studied. Cell-derived liposomes exhibited unilamellar morphology and were found to be of 100-200nm in diameter. Moreover, CCR5 that was expressed on the surface of the cell-derived liposomes was biologically active and correctly oriented. Cell-derived liposomes incubated with HIV-infected model cells exhibited significant and specific targeting to those gp120-expressing cells. To demonstrate the system efficacy, EDTA was selected as liposomal encapsulate and was shown to cause high cytotoxic effect when introduced into the cell cytoplasm. Finally, cell-derived liposomes containing EDTA led to a 60% reduction in the viability of gp120-expressing cells compared to no effect on control cells that do not express gp120. These results demonstrate the specific targeting and cytotoxic effect of CCR5-conjugated cell-derived liposomes towards gp120-expressing HIV model cells, suggesting for a potential new therapeutic approach. |
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Authors:
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Tomer Bronshtein; Naama Toledano; Dganit Danino; Shimon Pollack; Marcelle Machluf |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-26 |
Journal Detail:
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Title: Journal of controlled release : official journal of the Controlled Release Society Volume: - ISSN: 1873-4995 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-3-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8607908 Medline TA: J Control Release Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2010. Published by Elsevier B.V. |
Affiliation:
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Faculty of Biotechnology and Food Engineering, Technion - Israel Institute of technology, Haifa, Israel. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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