| Cell death-associated ADAMTS4 and versican degradation in vascular tissue. | |
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MedLine Citation:
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PMID: 19506088 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High blood flow through baboon polytetrafluorethylene aorto-iliac grafts increases neointimal vascular smooth muscle cell (SMC) death, neointimal atrophy, and cleavage of versican to generate the DPEAAE neoepitope, a marker of ADAMTS-mediated proteolysis. In this study, we have determined the effect of high blood flow on transcript abundance in the neointima for ADAMTS1, -4, -5, -8, -9, -15, and -20. We found that after 24 hr of flow, the mRNA for ADAMTS4 was significantly increased, whereas that for the other family members was unchanged. Because vascular SMC death is markedly increased in the graft after 24 hr of high flow, we next examined the possibility that the ADAMTS4 induction and the cell death are causally related. The addition of Fas ligand to SMC cultures increased both ADAMTS4 mRNA and cell death approximately 5-fold, consistent with the idea that ADAMTS4-dependent cleavage of versican may be partly responsible for cell death and tissue atrophy under these conditions. |
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Authors:
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Richard D Kenagy; Seung-Kee Min; Alexander W Clowes; John D Sandy |
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Publication Detail:
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Type: Journal Article Date: 2009-06-08 |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 57 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-18 Completed Date: 2009-09-15 Revised Date: 2010-09-02 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 889-97 Citation Subset: IM |
Affiliation:
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Center for Cardiovascular Biology, PO Box 358050, University of Washington School of Medicine, 815 Mercer St., Seattle, WA 98109. rkenagy@u.washington.edu. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ADAM Proteins
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biosynthesis*,
genetics Animals Aorta, Abdominal / metabolism, physiopathology Aorta, Thoracic / metabolism Atrophy Blood Vessel Prosthesis Cell Death Cells, Cultured Disease Models, Animal Fas Ligand Protein / pharmacology Humans Iliac Artery / physiopathology Male Muscle, Smooth, Vascular / metabolism*, pathology, physiopathology Myocytes, Smooth Muscle / metabolism*, pathology Papio cynocephalus Polytetrafluoroethylene RNA, Messenger / biosynthesis Regional Blood Flow Tunica Intima / metabolism, pathology Versicans / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fas Ligand Protein; 0/RNA, Messenger; 126968-45-4/Versicans; 9002-84-0/Polytetrafluoroethylene; EC 3.4.24.-/ADAM Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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