Document Detail


Cell cycle-regulated cortical dynein/dynactin promotes symmetric cell division by differential pole motion in anaphase.
MedLine Citation:
PMID:  22809624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In cultured mammalian cells, how dynein/dynactin contributes to spindle positioning is poorly understood. To assess the role of cortical dynein/dynactin in this process, we generated mammalian cell lines expressing localization and affinity purification (LAP)-tagged dynein/dynactin subunits from bacterial artificial chromosomes and observed asymmetric cortical localization of dynein and dynactin during mitosis. In cells with asymmetrically positioned spindles, dynein and dynactin were both enriched at the cortex distal to the spindle. NuMA, an upstream targeting factor, localized asymmetrically along the cell cortex in a manner similar to dynein and dynactin. During spindle motion toward the distal cortex, dynein and dynactin were locally diminished and subsequently enriched at the new distal cortex. At anaphase onset, we observed a transient increase in cortical dynein, followed by a reduction in telophase. Spindle motion frequently resulted in cells entering anaphase with an asymmetrically positioned spindle. These cells gave rise to symmetric daughter cells by dynein-dependent differential spindle pole motion in anaphase. Our results demonstrate that cortical dynein and dynactin dynamically associate with the cell cortex in a cell cycle-regulated manner and are required to correct spindle mispositioning in LLC-Pk1 epithelial cells.
Authors:
Elizabeth S Collins; Sai Keshavan Balchand; Jessica L Faraci; Patricia Wadsworth; Wei-Lih Lee
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-18
Journal Detail:
Title:  Molecular biology of the cell     Volume:  23     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-31     Completed Date:  2013-02-22     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3380-90     Citation Subset:  IM    
Affiliation:
Biology Department, University of Massachusetts, Amherst, MA 01003, USA.
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MeSH Terms
Descriptor/Qualifier:
Anaphase / physiology*
Animals
Cell Cycle Checkpoints
Cell Division / physiology*
Cell Line
Dyneins / metabolism*
Microtubule-Associated Proteins / metabolism*
Mitosis
Mitotic Spindle Apparatus / metabolism*
Nuclear Matrix-Associated Proteins / metabolism
Sus scrofa
Grant Support
ID/Acronym/Agency:
F32GM093602/GM/NIGMS NIH HHS; R01 GM076094/GM/NIGMS NIH HHS; R01GM076094/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Microtubule-Associated Proteins; 0/Nuclear Matrix-Associated Proteins; 144198-36-7/dynactin; EC 3.6.4.2/Dyneins
Comments/Corrections

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