Document Detail


Cell blebbing and membrane area homeostasis in spreading and retracting cells.
MedLine Citation:
PMID:  20858416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells remodel their plasma membrane and cytoskeleton during numerous physiological processes, including spreading and motility. Morphological changes require the cell to adjust its membrane tension on different timescales. While it is known that endo- and exocytosis regulate the cell membrane area in a timescale of 1 h, faster processes, such as abrupt cell detachment, require faster regulation of the plasma membrane tension. In this article, we demonstrate that cell blebbing plays a critical role in the global mechanical homeostasis of the cell through regulation of membrane tension. Abrupt cell detachment leads to pronounced blebbing (which slow detachment does not), and blebbing decreases with time in a dynamin-dependent fashion. Cells only start spreading after a lag period whose duration depends on the cell's blebbing activity. Our model quantitatively reproduces the monotonic decay of the blebbing activity and accounts for the lag phase in the spreading of blebbing cells.
Authors:
Leann L Norman; Jan Brugués; Jan Brugés; Kheya Sengupta; Pierre Sens; Helim Aranda-Espinoza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  99     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2011-01-04     Revised Date:  2012-04-26    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1726-33     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanics
Cattle
Cell Membrane / metabolism*
Cell Shape*
Cell Surface Extensions / metabolism*
Elasticity
Endocytosis
Endothelial Cells / cytology*
Homeostasis*
Models, Biological
Viscosity
Comments/Corrections
Erratum In:
Biophys J. 2010 Oct 20;99(8):2715
Note: Brugés, Jan [corrected to Brugués, Jan]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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