| Cell-based screening using high-throughput flow cytometry. | |
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MedLine Citation:
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PMID: 21050072 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This review describes the use of high-throughput flow cytometry for performing multiplexed cell-based and bead-based screens. With the many advances in cell-based analysis and screening, flow cytometry has historically been underutilized as a screening tool largely due to the limitations in handling large numbers of samples. However, there has been a resurgence in the use of flow cytometry due to a combination of innovations around instrumentation and a growing need for cell-based and bead-based applications. The HTFC™ Screening System (IntelliCyt Corporation, Albuquerque, NM) is a novel flow cytometry-based screening platform that incorporates a fast sample-loading technology, HyperCyt®, with a two-laser, six-parameter flow cytometer and powerful data analysis capabilities. The system is capable of running multiplexed screening assays at speeds of up to 40 wells per minute, enabling the processing of a 96- and 384-well plates in as little as 3 and 12 min, respectively. Embedded in the system is HyperView®, a data analysis software package that allows rapid identification of hits from multiplexed high-throughput flow cytometry screening campaigns. In addition, the software is incorporated into a server-based data management platform that enables seamless data accessibility and collaboration across multiple sites. High-throughput flow cytometry using the HyperCyt technology has been applied to numerous assay areas and screening campaigns, including efflux transporters, whole cell and receptor binding assays, functional G-protein-coupled receptor screening, in vitro toxicology, and antibody screening. |
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Authors:
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Christopher B Black; Thomas D Duensing; Linda S Trinkle; R Terry Dunlay |
Publication Detail:
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Type: Journal Article; Review Date: 2010-11-04 |
Journal Detail:
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Title: Assay and drug development technologies Volume: 9 ISSN: 1557-8127 ISO Abbreviation: Assay Drug Dev Technol Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-14 Completed Date: 2011-06-02 Revised Date: 2011-08-25 |
Medline Journal Info:
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Nlm Unique ID: 101151468 Medline TA: Assay Drug Dev Technol Country: United States |
Other Details:
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Languages: eng Pagination: 13-20 Citation Subset: IM |
Affiliation:
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IntelliCyt Corporation, 317 Commercial St. NE, Albuquerque, NM 87102, USA. cblack@intellicyt.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Assay
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instrumentation* Cell Physiological Phenomena / drug effects* Drug Evaluation, Preclinical / instrumentation* Equipment Design Equipment Failure Analysis Flow Cytometry / instrumentation* Flow Injection Analysis / instrumentation* High-Throughput Screening Assays / instrumentation* Microfluidic Analytical Techniques / instrumentation* |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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