| Cell-based microarrays of infectious adenovirus encoding short hairpin RNA (shRNA). | |
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MedLine Citation:
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PMID: 21104057 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Genome-wide screening procedures have developed into a useful tool for assigning new functions to known proteins or for identifying new interplayers in cell metabolism, especially under pathological conditions. Since primary cells reflect the physiological situation more closely than transformed cell lines, their employment in such screenings is highly desirable. A difficulty to overcome--besides the shortage in cell supply--is that primary cells are less amenable to classical methods of genetic manipulation such as lipofection or electroporation. By using adenovirus as the vehicle for genetic manipulation, efficient transfer of genetic information can be achieved without drastic effects on cell viability, and by using a microarray format even small amounts of cells suffice to perform a screen. |
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Authors:
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Hansjürgen Volkmer; Frank Weise |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 706 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2011 |
Date Detail:
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Created Date: 2010-11-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 97-106 Citation Subset: IM |
Affiliation:
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Natural and Medical Sciences Institute, University of Tübingen, Reutlingen, Germany. volkmer@nmi.de |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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