Document Detail


Cell-based microarrays of infectious adenovirus encoding short hairpin RNA (shRNA).
MedLine Citation:
PMID:  21104057     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Genome-wide screening procedures have developed into a useful tool for assigning new functions to known proteins or for identifying new interplayers in cell metabolism, especially under pathological conditions. Since primary cells reflect the physiological situation more closely than transformed cell lines, their employment in such screenings is highly desirable. A difficulty to overcome--besides the shortage in cell supply--is that primary cells are less amenable to classical methods of genetic manipulation such as lipofection or electroporation. By using adenovirus as the vehicle for genetic manipulation, efficient transfer of genetic information can be achieved without drastic effects on cell viability, and by using a microarray format even small amounts of cells suffice to perform a screen.
Authors:
Hansjürgen Volkmer; Frank Weise
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  706     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2010-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-106     Citation Subset:  IM    
Affiliation:
Natural and Medical Sciences Institute, University of Tübingen, Reutlingen, Germany. volkmer@nmi.de
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