Document Detail


A cell-based assay for screening spindle checkpoint inhibitors.
MedLine Citation:
PMID:  22352901     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In eukaryotes, the spindle checkpoint acts as a surveillance mechanism that ensures faithful chromosome segregation. The spindle checkpoint prevents premature separation of sister chromatids and the onset of anaphase until every chromosome is properly attached to the mitotic spindle. Tumorigenesis might result from generation of aneuploidy by dysfunction of the spindle checkpoint. Differences of the checkpoint system in normal cells versus tumor cells might provide a new opportunity in cancer drug development; therefore, efforts to identify the spindle checkpoint inhibitors have been fostered. Based on spindle checkpoint inhibitors being able to induce cells to exit mitotic arrest caused by microtubule drug treatment, we developed a cell-based assay to screen compounds that were potential spindle checkpoint inhibitors. This assay was validated with a known spindle checkpoint inhibitor and was easy to adapt to a large-scale screening. It also had the advantages of being high in sensitivity and low in cost.
Authors:
Zhen Hua Wu; Long Yu Hu; Da Qian Xu; Xiaotong Li
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-21
Journal Detail:
Title:  Assay and drug development technologies     Volume:  10     ISSN:  1557-8127     ISO Abbreviation:  Assay Drug Dev Technol     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-22     Completed Date:  2012-12-31     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  101151468     Medline TA:  Assay Drug Dev Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  344-52     Citation Subset:  IM    
Affiliation:
School of Life Sciences, Xiamen University, Xiamen, Fujian, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Blotting, Western
Cell Cycle / drug effects
Cell Cycle Checkpoints / drug effects*
Cell Line
Coloring Agents
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical / methods*
Drug Screening Assays, Antitumor / methods*
Enzyme Inhibitors / pharmacology
Fluorescent Antibody Technique
HeLa Cells
High-Throughput Screening Assays / methods*
Humans
Mitotic Index
Mitotic Spindle Apparatus / drug effects*
Nocodazole / pharmacology
Protein-Serine-Threonine Kinases / antagonists & inhibitors
Purines / pharmacology
Tetrazolium Salts
Thiazoles
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Coloring Agents; 0/Enzyme Inhibitors; 0/Purines; 0/Tetrazolium Salts; 0/Thiazoles; 0/roscovitine; 298-93-1/thiazolyl blue; 31430-18-9/Nocodazole; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/aurora kinase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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