| A cell-based assay for screening spindle checkpoint inhibitors. | |
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MedLine Citation:
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PMID: 22352901 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In eukaryotes, the spindle checkpoint acts as a surveillance mechanism that ensures faithful chromosome segregation. The spindle checkpoint prevents premature separation of sister chromatids and the onset of anaphase until every chromosome is properly attached to the mitotic spindle. Tumorigenesis might result from generation of aneuploidy by dysfunction of the spindle checkpoint. Differences of the checkpoint system in normal cells versus tumor cells might provide a new opportunity in cancer drug development; therefore, efforts to identify the spindle checkpoint inhibitors have been fostered. Based on spindle checkpoint inhibitors being able to induce cells to exit mitotic arrest caused by microtubule drug treatment, we developed a cell-based assay to screen compounds that were potential spindle checkpoint inhibitors. This assay was validated with a known spindle checkpoint inhibitor and was easy to adapt to a large-scale screening. It also had the advantages of being high in sensitivity and low in cost. |
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Authors:
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Zhen Hua Wu; Long Yu Hu; Da Qian Xu; Xiaotong Li |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-02-21 |
Journal Detail:
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Title: Assay and drug development technologies Volume: 10 ISSN: 1557-8127 ISO Abbreviation: Assay Drug Dev Technol Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-08-22 Completed Date: 2012-12-31 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 101151468 Medline TA: Assay Drug Dev Technol Country: United States |
Other Details:
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Languages: eng Pagination: 344-52 Citation Subset: IM |
Affiliation:
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School of Life Sciences, Xiamen University, Xiamen, Fujian, P.R. China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Blotting, Western Cell Cycle / drug effects Cell Cycle Checkpoints / drug effects* Cell Line Coloring Agents Dose-Response Relationship, Drug Drug Evaluation, Preclinical / methods* Drug Screening Assays, Antitumor / methods* Enzyme Inhibitors / pharmacology Fluorescent Antibody Technique HeLa Cells High-Throughput Screening Assays / methods* Humans Mitotic Index Mitotic Spindle Apparatus / drug effects* Nocodazole / pharmacology Protein-Serine-Threonine Kinases / antagonists & inhibitors Purines / pharmacology Tetrazolium Salts Thiazoles |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Coloring Agents; 0/Enzyme Inhibitors; 0/Purines; 0/Tetrazolium Salts; 0/Thiazoles; 0/roscovitine; 298-93-1/thiazolyl blue; 31430-18-9/Nocodazole; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/aurora kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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