Document Detail

Cecal ligation and incision: an acute onset model of severe sepsis in rats.
MedLine Citation:
PMID:  18486152     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Sepsis is a leading cause of death among critically ill patients. Up to now, severe sepsis with acute onset in animals has been induced mainly through injection of single bacteria species or endotoxin and not through a surgical procedure, which might adequately mirror the situation in septic patients. We therefore aimed to establish a surgical model of severe sepsis in rodents fulfilling international sepsis criteria. MATERIALS AND METHODS: Twenty-eight anesthetized/ventilated Sprague Dawley rats underwent laparotomy and cecal mobilization. The cecum was either replaced into the abdomen (SHAM, n = 14) or the cecum and the mesenteric blood vessels were ligated, and the cecum was opened through a 1.5 cm blade incision (cecal ligation and incision, CLI, n = 14). RESULTS: Within 390 min, mortality was 0% (SHAM) and 50% (CLI), respectively. Compared with SHAM, CLI resulted in a 43% reduction of mean arterial blood pressure and in severe metabolic acidosis as measured by arterial base excess and pH. CLI led to a 15-fold increase in mononuclear cell population and to a 5-fold accumulation of nitrite in peritoneal lavage. Abdominal swabs from the Douglas cavity in CLI-animals showed gram-positive and gram-negative bacterial growth on agar compared with sterile swabs from SHAM-animals. In CLI-animals, plasma IL-1beta level was increased to 435 pg/mL (SHAM: 10 pg/mL) and plasma IL-6 level to 19718 pg/mL (SHAM: 832 pg/mL). CONCLUSIONS: CLI causes bacterial peritonitis with subsequent systemic inflammation and organ dysfunction. Thus, CLI mimics clinical sepsis and provides a surgical short term model of severe sepsis in rodents.
Patrick Scheiermann; Sandra Hoegl; Marc Revermann; Devan Ahluwalia; Johannes Zander; Kim A Boost; Thach Nguyen; Bernhard Zwissler; Heiko Muhl; Christian Hofstetter
Publication Detail:
Type:  Journal Article     Date:  2008-03-18
Journal Detail:
Title:  The Journal of surgical research     Volume:  151     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  132-7     Citation Subset:  IM    
Department of Anesthesiology, Intensive Care Medicine, and Pain Therapy, Hospital of the Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
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MeSH Terms
Acidosis / physiopathology
Acute Disease
Blood Pressure / physiology
Body Temperature / physiology
Cecum / surgery*
Disease Models, Animal*
Heart Rate / physiology
Hydrogen-Ion Concentration
Interleukin-1beta / blood
Interleukin-6 / blood
Peritoneum / microbiology
Peritonitis / microbiology*,  physiopathology
Rats, Sprague-Dawley
Sepsis / microbiology*,  physiopathology
Tidal Volume / physiology
Reg. No./Substance:
0/Interleukin-1beta; 0/Interleukin-6

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