| Cdk9-55: a new player in muscle regeneration. | |
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MedLine Citation:
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PMID: 18546201 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adult skeletal muscle contains a specialized population of myogenic quiescent stem cells, termed satellite cells, which contribute to repair myofibers after injury. During muscle regeneration, satellite cells exit their normal quiescent state, proliferate, activating MyoD and Myf-5 expression, and finally differentiate and fuse to reconstitute the injured muscle architecture. We have previously reported that cdk9 is required for myogenesis in vitro by activating MyoD-dependent transcription. In myoblasts induced to differentiate, MyoD recruits cdk9 on the chromatin of muscle-specific regulatory regions. This event correlates with chromatin-modifying enzyme recruitment and phosphorylation of cdk9-specific target residues at the carboxyl-terminal domain of RNA polymerase II. Here we report that a second cdk9 isoform, termed cdk9-55, plays a fundamental role in muscle regeneration and differentiation in vivo. This alternative form is specifically induced in injured myofibers and its activity is strictly required for the completion of muscle regeneration process. |
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Authors:
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Cristina Giacinti; Antonio Musarò; Giulia De Falco; Isabelle Jourdan; Mario Molinaro; Luigi Bagella; Cristiano Simone; Antonio Giordano |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular physiology Volume: 216 ISSN: 1097-4652 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-06-23 Completed Date: 2008-08-26 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 576-82 Citation Subset: IM |
Affiliation:
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Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation / physiology Cells, Cultured Cyclin-Dependent Kinase 9 / genetics, metabolism* Humans Mice Mice, Inbred C57BL Muscle Development / physiology* Muscle Fibers, Skeletal / cytology, metabolism* Muscle, Skeletal* / cytology, physiology MyoD Protein / genetics, metabolism Protein Isoforms / genetics, metabolism* Regeneration / physiology* Satellite Cells, Skeletal Muscle / cytology, metabolism Stem Cells / cytology, physiology* |
| Chemical | |
Reg. No./Substance:
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0/MyoD Protein; 0/Protein Isoforms; EC 2.7.11.22/Cyclin-Dependent Kinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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