| Cdk6-cyclin D3 complex evades inhibition by inhibitor proteins and uniquely controls cell's proliferation competence. | |
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MedLine Citation:
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PMID: 11360184 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mammalian cells require a cyclin D-dependent kinase for the cell cycle start, yet many mesenchymal cells express three seemingly redundant D cyclins and similarly, seemingly redundant Cdk4 and Cdk6 as their kinase partners. We have found that the Cdk6-cyclin D3 complex is unique among the D cyclin and kinase combinations in the ability to promote the cell cycle start. In an anchorage-minus G(1)-arrested rat fibroblast, only Cdk6-D3 retains kinase activity due mainly to its ability to evade inhibition by p27(KIP1) and p21(CIP1) with a resemblance to viral cyclin-bound Cdk6. Rodent fibroblasts engineered to overexpress both Cdk6 and cyclin D3 highly resist serum starvation- or cell-cell contact-imposed G(1)-arrest. In BALB/c 3T3 cells, D3 is constitutively expressed, but Cdk6 is markedly induced with concomitant activation upon stimulation with a growth-promoting factor. These results suggest a role for the Cdk6-D3 complex in regulating cell's proliferation ability in response to external stimuli. |
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Authors:
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J Lin; S Jinno; H Okayama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncogene Volume: 20 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-05-21 Completed Date: 2001-05-31 Revised Date: 2012-06-25 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
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Languages: eng Pagination: 2000-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Cell Adhesion / physiology Cell Cycle Proteins* Cell Division / physiology Contact Inhibition / physiology Cyclin D3 Cyclin-Dependent Kinase 6 Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Cyclin-Dependent Kinases* Cyclins / antagonists & inhibitors, metabolism, physiology* G1 Phase / physiology Mice Mice, Inbred BALB C Mice, Inbred C3H Microtubule-Associated Proteins / metabolism, physiology Platelet-Derived Growth Factor / pharmacology Precipitin Tests Protein-Serine-Threonine Kinases / antagonists & inhibitors, metabolism, physiology* Rats Tetradecanoylphorbol Acetate / pharmacology Tumor Suppressor Proteins* |
| Chemical | |
Reg. No./Substance:
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0/Ccnd3 protein, mouse; 0/Ccnd3 protein, rat; 0/Cdkn1a protein, mouse; 0/Cdkn1a protein, rat; 0/Cdkn1b protein, mouse; 0/Cdkn1b protein, rat; 0/Cell Cycle Proteins; 0/Cyclin D3; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Microtubule-Associated Proteins; 0/Platelet-Derived Growth Factor; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 16561-29-8/Tetradecanoylphorbol Acetate; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/Cdk6 protein, mouse; EC 2.7.11.22/Cdk6 protein, rat; EC 2.7.11.22/Cyclin-Dependent Kinase 6; EC 2.7.11.22/Cyclin-Dependent Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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