| Cdk1-dependent phosphorylation of Cdc13 coordinates telomere elongation during cell-cycle progression. | |
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MedLine Citation:
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PMID: 19135888 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Elongation of telomeres by telomerase replenishes the loss of terminal telomeric DNA repeats during each cell cycle. In budding yeast, Cdc13 plays an essential role in telomere length homeostasis, partly through its interactions with both the telomerase complex and the competing Stn1-Ten1 complex. Previous studies in yeast have shown that telomere elongation by telomerase is cell cycle dependent, but the mechanism underlying this dependence is unclear. In S. cerevisiae, a single cyclin-dependent kinase Cdk1 (Cdc28) coordinates the serial events required for the cell division cycle, but no Cdk1 substrate has been identified among telomerase and telomere-associated factors. Here we show that Cdk1-dependent phosphorylation of Cdc13 is essential for efficient recruitment of the yeast telomerase complex to telomeres by favoring the interaction of Cdc13 with Est1 rather than the competing Stn1-Ten1 complex. These results provide a direct mechanistic link between coordination of telomere elongation and cell-cycle progression in vivo. |
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Authors:
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Shang Li; Svetlana Makovets; Tetsuya Matsuguchi; Justin D Blethrow; Kevan M Shokat; Elizabeth H Blackburn |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell Volume: 136 ISSN: 1097-4172 ISO Abbreviation: Cell Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-12 Completed Date: 2009-01-26 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
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Languages: eng Pagination: 50-61 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, University of California, San Francisco, Box 2200, San Francisco, CA 94143-2200, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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CDC28 Protein Kinase, S cerevisiae
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metabolism* Cell Cycle* Phosphorylation Saccharomyces cerevisiae / cytology*, metabolism* Saccharomyces cerevisiae Proteins / metabolism* Telomerase / metabolism Telomere / metabolism* Telomere-Binding Proteins / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM026259-31/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cdc13 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Telomere-Binding Proteins; EC 2.7.11.22/CDC28 Protein Kinase, S cerevisiae; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
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