Document Detail


Cdc7-Dbf4 is a gene-specific regulator of meiotic transcription in yeast.
MedLine Citation:
PMID:  22106412     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Meiosis divides the chromosome number of the cell in half by having two rounds of chromosome segregation follow a single round of chromosome duplication. The first meiotic division is unique in that homologous pairs of sister chromatids segregate to opposite poles. Recent work in budding and fission yeast has shown that the cell cycle kinase, Cdc7-Dbf4, is required for many meiosis-specific chromosomal functions necessary for proper disjunction at meiosis I. This work reveals another role for Cdc7 in meiosis as a gene-specific regulator of the global transcription factor, Ndt80, which is required for exit from pachytene and entry into the meiotic divisions in budding yeast. Cdc7-Dbf4 promotes NDT80 transcription by relieving repression mediated by a complex of Sum1, Rfm1, and a histone deacetylase, Hst1. Sum1 exhibits meiosis-specific Cdc7-dependent phosphorylation, and mass spectrometry analysis reveals a dynamic and complex pattern of phosphorylation events, including four constitutive cyclin-dependent kinase (Cdk1) sites and 11 meiosis-specific Cdc7-Dbf4-dependent sites. Analysis of various phosphorylation site mutants suggests that Cdc7 functions with both Cdk1 and the meiosis-specific kinase Ime2 to control this critical transition point during meiosis.
Authors:
Hsiao-Chi Lo; Ryan C Kunz; Xiangyu Chen; Allison Marullo; Steven P Gygi; Nancy M Hollingsworth
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-11-21
Journal Detail:
Title:  Molecular and cellular biology     Volume:  32     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-28     Completed Date:  2012-02-21     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  541-57     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cell Cycle Proteins / genetics,  metabolism*
DNA-Binding Proteins / genetics*,  metabolism
Gene Expression Regulation, Fungal*
Meiosis
Mitogen-Activated Protein Kinases / genetics,  metabolism
Molecular Sequence Data
Nuclear Proteins / chemistry,  genetics,  metabolism
Phosphorylation
Promoter Regions, Genetic
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Repressor Proteins / chemistry,  genetics,  metabolism
Saccharomyces cerevisiae / cytology*,  genetics*,  metabolism
Saccharomyces cerevisiae Proteins / chemistry,  genetics*,  metabolism*
Sirtuin 2 / genetics,  metabolism
Transcription Factors / genetics*,  metabolism
Transcriptional Activation
Grant Support
ID/Acronym/Agency:
5P01GM088297/GM/NIGMS NIH HHS; HG3456/HG/NHGRI NIH HHS; R01GM50717/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Dbf4 protein, S cerevisiae; 0/IME1 protein, S cerevisiae; 0/NDT80 protein, S cerevisiae; 0/Nuclear Proteins; 0/Repressor Proteins; 0/Rfm1 protein, S cerevisiae; 0/SUM1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; EC 2.7.1.-/CDC7 protein, S cerevisiae; EC 2.7.1.-/Smk1 protein, S cerevisiae; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 3.5.1.-/HST1 protein, S cerevisiae; EC 3.5.1.-/Sirtuin 2
Comments/Corrections

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