Document Detail


Cdc42p and Fus2p act together late in yeast cell fusion.
MedLine Citation:
PMID:  22323294     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell fusion is the key event of fertilization that gives rise to the diploid zygote and is a nearly universal aspect of eukaryotic biology. In the yeast Saccharomyces cerevisiae, several mutants have been identified that are defective for cell fusion, and yet the molecular mechanism of this process remains obscure. One obstacle has been that genetic screens have mainly focused on mating-specific factors, whereas the process likely involves housekeeping proteins as well. Here we implicate Cdc42p, an essential protein with roles in multiple aspects of morphogenesis, as a core component of the yeast cell fusion pathway. We identify a point mutant in the Rho-insert domain of CDC42, called cdc42-138, which is specifically defective in cell fusion. The cell fusion defect is not a secondary consequence of ineffective signaling or polarization. Genetic and morphological data show that Cdc42p acts at a late stage in cell fusion in concert with a key cell fusion regulator, Fus2p, which contains a Dbl-homology domain. We find that Fus2p binds specifically with activated Cdc42p, and binding is blocked by the cdc42-138 mutation. Thus, in addition to signaling and morphogenetic roles in mating, Cdc42p plays a role late in cell fusion via activation of Fus2p.
Authors:
Casey A Ydenberg; Richard A Stein; Mark D Rose
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-02-09
Journal Detail:
Title:  Molecular biology of the cell     Volume:  23     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-30     Completed Date:  2012-09-13     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1208-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Polarity
Cytoskeletal Proteins / chemistry,  genetics,  metabolism*
Genes, Fungal
Guanosine Triphosphate / metabolism
Membrane Proteins / chemistry,  genetics,  metabolism*
Microscopy, Electron, Transmission
Models, Molecular
Peptides / metabolism
Pheromones / metabolism
Point Mutation
Protein Interaction Domains and Motifs
Recombinant Fusion Proteins / chemistry,  genetics,  metabolism
Saccharomyces cerevisiae / cytology,  genetics,  physiology*
Saccharomyces cerevisiae Proteins / chemistry,  genetics,  metabolism*
Signal Transduction
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / chemistry,  genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
GM37739/GM/NIGMS NIH HHS; R01 GM037739/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cytoskeletal Proteins; 0/FUS2 protein, S cerevisiae; 0/Membrane Proteins; 0/Peptides; 0/Pheromones; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; 61194-02-3/mating factor; 86-01-1/Guanosine Triphosphate; EC 3.6.5.2/cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Comments/Corrections

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