Document Detail

Cdc42-dependent localization of polarisome component Spa2 to the incipient bud site is independent of the GDP/GTP exchange factor Cdc24.
MedLine Citation:
PMID:  16325503     Owner:  NLM     Status:  MEDLINE    
Cdc42, a member of the Rho subfamily of small GTPases, is highly conserved in both sequence and function across eukaryotic species. In budding yeast, Cdc42 triggers polarized growth necessary for bud emergence via rearrangement of the actin cytoskeleton. It has been shown that the role of Cdc42 in bud emergence requires both Cdc28-Cln (G1) kinase and the passage through START. In this report, we show that Cdc42 also serves an essential function in the establishment of bud site prior to START by catalyzing the translocation of bud-site components such as Spa2 to the cell cortex. Our analysis of various conditional alleles of CDC42 suggests that these two functions (bud site establishment and bud emergence) are genetically separable. Surprisingly, the role of Cdc42 in the cortical localization of Spa2 appears to be independent of its well known GTP/GDP exchange factor Cdc24. We also provide evidence that this role of Cdc42 requires the function of the COPI coatomer complex.
Padmashree C G Rida; Uttam Surana
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-30
Journal Detail:
Title:  European journal of cell biology     Volume:  84     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-05     Completed Date:  2006-03-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  939-49     Citation Subset:  IM    
Institute of Molecular and Cell Biology, Proteos, Republic of Singapore.
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MeSH Terms
Cell Cycle / physiology
Cell Cycle Proteins / genetics,  metabolism*
Cell Nucleus / chemistry
Cell Polarity
Coat Protein Complex I / physiology
Cytoskeletal Proteins
Cytoskeleton / physiology
Gene Expression Regulation, Fungal
Genes, Fungal
Guanine Nucleotide Exchange Factors / genetics,  metabolism*
Microscopy, Confocal
Saccharomyces cerevisiae / cytology,  growth & development,  physiology*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / genetics,  physiology*
Reg. No./Substance:
0/CDC24 protein, S cerevisiae; 0/Cell Cycle Proteins; 0/Coat Protein Complex I; 0/Cytoskeletal Proteins; 0/Guanine Nucleotide Exchange Factors; 0/SPA2 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC GTP-Binding Protein, Saccharomyces cerevisiae

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