Document Detail

Cdc42 GTPase and Rac1 GTPase act downstream of p120 catenin and require GTP exchange during gastrulation of zebrafish mesoderm.
MedLine Citation:
PMID:  22911626     Owner:  NLM     Status:  Publisher    
We investigated the roles of p120 catenin, Cdc42, Rac1, and RhoA GTPases in regulating migration of presomitic mesoderm cells in zebrafish embryos. p120 catenin has dual roles: It binds the intracellular and juxtamembrane region of cadherins to stabilize cadherin-mediated adhesion with the aid of RhoA GTPase, and it activates Cdc42 GTPase and Rac1 GTPase in the cytosol to initiate cell motility. During gastrulation of zebrafish embryos, knockdown of the synthesis of zygotic p120 catenin?δ1 mRNAs with a splice-site morpholino caused lateral widening and anterior-posterior shortening of the presomitic mesoderm and somites and a shortened anterior-posterior axis: these phenotypes indicate a cell-migration defect. Co-injection of low amounts of wild-type Cdc42 or wild-type Rac1 or dominant-negative RhoA mRNAs, but not constitutively-active Cdc42 mRNA, rescued these p120 catenin δ1-depleted embryos. These downstream small GTPases require appropriate spatiotemporal stimulation or cycling of GTP to guide mesodermal cell migration. A delicate balance of Rho GTPases and p120 catenin underlies normal development.
Cynthia L Hsu; Claire P Muerdter; Abhay D Knickerbocker; Ryan M Walsh; Martha A Zepeda-Rivera; Kevin H Depner; Maya Sangesland; Trinidad B Cisneros; Ju Youn Kim; Patricia Sanchez-Vazquez; Lidia Cherezova; Rainy D Regan; Nadia M Bahrami; Elizabeth A Gray; Andrew Y Chan; Terry Chen; Milly Y Rao; Merrill B Hille
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-21
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  -     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Department of Biology, Box 351800, University of Washington, Seattle WA, 98195-1800, USA.
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