Document Detail

Cd2+ responses of cultured human blood cells.
MedLine Citation:
PMID:  6868086     Owner:  NLM     Status:  MEDLINE    
Cd2+ cytotoxicity, uptake, and partitioning, and Cd2+-induced metallothioneine synthesis were studied in cultured peripheral human blood cells. Mononuclear cells were found to resist relatively high levels of Cd2+. Few cells were killed below 50 microM Cd2+. Above this value, survival decreased exponentially with dose. The mean LD50 for mononuclear cells cultured in Cd2+ for 40 hr was 100 microM. Polymorphonuclear cells (granulocytes) were found to be more resistant, with a significantly higher threshold and LD50, and a more complex dose response. Most of the Cd2+ incorporated by blood cells was taken up by nucleated cells. Despite their greater resistance, polymorphonuclear cells incorporated more Cd2+ at higher doses (50 to 150 microM) than did mononuclear cells. No Cd2+ was bound to metallothioneine in polymorphonuclear cells following exposure to Cd2+ for even extended periods of time (18 hr) at high doses of 109Cd2+ (25 microM). Instead Cd2+ appeared in a Sephadex G-75 peak of approximately 60,000 Da, as well as in the void peak. No significant amount of preexisting metallothioneine (MT) or metallothioneine mRNA was found in the mononuclear cells. However, MT synthesis was induced rapidly following exposure to Cd2+. [109Cd2+]MT appeared within 1 hr following exposure to 50 microM 109Cd2+, and MT synthesis rates measured from [35S]cysteine incorporation were found to be maximal within 4 hr.
M D Enger; C E Hildebrand; C C Stewart
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  69     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-08-26     Completed Date:  1983-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  214-24     Citation Subset:  IM    
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MeSH Terms
Cadmium / blood*,  pharmacology
Cell Survival / drug effects
Cells, Cultured
Cysteine / metabolism
Granulocytes / metabolism
Leukocytes / drug effects,  metabolism*
Lymphocytes / metabolism
Metallothionein / biosynthesis
Monocytes / metabolism
Reg. No./Substance:
52-90-4/Cysteine; 7440-43-9/Cadmium; 9038-94-2/Metallothionein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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