| The CcrM DNA methyltransferase of Agrobacterium tumefaciens is essential, and its activity is cell cycle regulated. | |
| | |
MedLine Citation:
|
PMID: 11325934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
DNA methylation is now recognized as a regulator of multiple bacterial cellular processes. CcrM is a DNA adenine methyltransferase found in the alpha subdivision of the proteobacteria. Like the Dam enzyme, which is found primarily in Escherichia coli and other gamma proteobacteria, it does not appear to be part of a DNA restriction-modification system. The CcrM homolog of Agrobacterium tumefaciens was found to be essential for viability. Overexpression of CcrM is associated with significant abnormalities of cell morphology and DNA ploidy. Mapping of the transcriptional start site revealed a conserved binding motif for the global response regulator CtrA at the -35 position; this motif was footprinted by purified Caulobacter crescentus CtrA protein in its phosphorylated state. We have succeeded in isolating synchronized populations of Agrobacterium cells and analyzing their progression through the cell cycle. We demonstrate that DNA replication and cell division can be followed in an orderly manner and that flagellin expression is cyclic, consistent with our observation that motility varies during the cell cycle. Using these synchronized populations, we show that CcrM methylation of the chromosome is restricted to the late S phase of the cell cycle. Thus, within the alpha subdivision, there is a conserved cell cycle dependence and regulatory mechanism controlling ccrM expression. |
| | |
Authors:
|
L S Kahng; L Shapiro |
Related Documents
:
|
11823794 - Sibling rivalry in the e2f family. 22302684 - Increased radioresistance via g12s k-ras by compensatory upregulation of mapk and pi3k ... 18946044 - A bacterial control circuit integrates polar localization and proteolysis of key regula... 19345744 - Downregulation of neutral ceramidase by gemcitabine: implications for cell cycle regula... 19431334 - Passive electrical properties of microorganisms: i. conductivity of escherichia coli an... 22532134 - Postnatal changes in testicular development and androgen receptors immunolocalization i... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of bacteriology Volume: 183 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2001 May |
Date Detail:
|
Created Date: 2001-04-30 Completed Date: 2001-05-31 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
|
Languages: eng Pagination: 3065-75 Citation Subset: IM |
Affiliation:
|
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/AF327563 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Base Sequence Cell Cycle / physiology* Culture Media DNA Methylation* Flow Cytometry Gene Expression Regulation, Bacterial* Molecular Sequence Data Promoter Regions, Genetic Rhizobium radiobacter / enzymology*, growth & development, physiology Site-Specific DNA-Methyltransferase (Adenine-Specific) / chemistry, genetics*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
GM51426/GM/NIGMS NIH HHS; K08-AI-01510/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; EC 2.1.1.-/DNA modification methylase CcrM; EC 2.1.1.72/Site-Specific DNA-Methyltransferase (Adenine-Specific) |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Contributions of PBP 5 and DD-carboxypeptidase penicillin binding proteins to maintenance of cell sh...
Next Document: Role of Escherichia coli nitrogen regulatory genes in the nitrogen response of the Azotobacter vinel...