| Cbl-associated protein regulates assembly and function of two tension-sensing structures in Drosophila. | |
| | |
MedLine Citation:
|
PMID: 23293294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cbl-associated protein (CAP) localizes to focal adhesions and associates with numerous cytoskeletal proteins; however, its physiological roles remain unknown. Here, we demonstrate that Drosophila CAP regulates the organization of two actin-rich structures in Drosophila: muscle attachment sites (MASs), which connect somatic muscles to the body wall; and scolopale cells, which form an integral component of the fly chordotonal organs and mediate mechanosensation. Drosophila CAP mutants exhibit aberrant junctional invaginations and perturbation of the cytoskeletal organization at the MAS. CAP depletion also results in collapse of scolopale cells within chordotonal organs, leading to deficits in larval vibration sensation and adult hearing. We investigate the roles of different CAP protein domains in its recruitment to, and function at, various muscle subcellular compartments. Depletion of the CAP-interacting protein Vinculin results in a marked reduction in CAP levels at MASs, and vinculin mutants partially phenocopy Drosophila CAP mutants. These results show that CAP regulates junctional membrane and cytoskeletal organization at the membrane-cytoskeletal interface of stretch-sensitive structures, and they implicate integrin signaling through a CAP/Vinculin protein complex in stretch-sensitive organ assembly and function. |
| | |
Authors:
|
Rajnish Bharadwaj; Madhuparna Roy; Tomoko Ohyama; Elena Sivan-Loukianova; Michael Delannoy; Thomas E Lloyd; Marta Zlatic; Daniel F Eberl; Alex L Kolodkin |
Related Documents
:
|
22941504 - Distribution, formation and regulation of gas vesicles. 23125894 - Protein oxidative damage at the crossroads of cellular senescence, aging, and age-relat... 22994494 - Peroxisome assembly and functional diversity in eukaryotic microorganisms. 23070364 - Mammalian proapoptotic factor chac1 and its homologues function as γ-glutamyl cyclotra... 14693804 - Prediction of protein subcellular locations using fuzzy k-nn method. 11111094 - Molecular biology and pathophysiological aspects of plasma cholesteryl ester transfer p... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 140 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2013 Feb |
Date Detail:
|
Created Date: 2013-01-07 Completed Date: 2013-03-07 Revised Date: 2013-05-13 |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
|
Languages: eng Pagination: 627-38 Citation Subset: IM |
Affiliation:
|
Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Actin Cytoskeleton
/
metabolism,
physiology Amino Acid Sequence Animal Structures / metabolism, physiology*, ultrastructure Animals Binding Sites Cell Membrane / metabolism, physiology Cell-Matrix Junctions / metabolism, physiology Cytoskeletal Proteins / genetics, metabolism* Drosophila / anatomy & histology, genetics, metabolism, physiology* Electrophysiological Phenomena Gene Expression Regulation, Developmental* Genome, Insect Hearing Disorders / genetics, pathology, veterinary Integrins / metabolism Larva / genetics, metabolism, physiology, ultrastructure Mechanotransduction, Cellular Microscopy, Electron, Transmission Multiprotein Complexes / genetics, metabolism Muscles / cytology, metabolism Protein Interaction Mapping Sequence Homology, Amino Acid Signal Transduction Talin / genetics, metabolism Vibration Vinculin / genetics, metabolism src Homology Domains |
| Grant Support | |
ID/Acronym/Agency:
|
NS35165/NS/NINDS NIH HHS; P30 DC010362/DC/NIDCD NIH HHS; R01 DC004848/DC/NIDCD NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
|
0/Cytoskeletal Proteins; 0/Integrins; 0/Multiprotein Complexes; 0/Talin; 125361-02-6/Vinculin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Kif5b controls the localization of myofibril components for their assembly and linkage to the myoten...
Next Document: Sox17 haploinsufficiency results in perinatal biliary atresia and hepatitis in C57BL/6 background mi...