| Caveolin 3-mediated integrin {beta}1 signaling is required for the proliferation of folliculo-stellate cells in rat anterior pituitary gland under the influence of extracellular matrix. | |
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MedLine Citation:
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PMID: 21508095 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Folliculo-stellate (FS) cells in the anterior pituitary gland are believed to have multifunctional properties. Using transgenic rats that express green fluorescent protein (GFP) specifically in FS cells in the anterior pituitary gland (S100b-GFP rats), we recently revealed that FS cells in primary culture exhibited marked proliferation in the presence of laminin, an extracellular matrix (ECM) component of the basement membrane. In a process referred to as matricrine action, FS cells receive ECM as a signal through their receptors, which results in morphological and functional changes. In the present study, we investigated matricrine signaling in FS cells and observed that the proliferation of FS cells is mediated by integrin β1, which is involved in various signaling pathways for cell migration and proliferation in response to ECM. Next, we analyzed downstream events of the integrin β1 signaling pathway in the proliferation of FS cells and identified caveolin 3 as a potential candidate molecule. Caveolin 3 is a membrane protein that binds cholesterol and a number of signaling molecules that interact with integrin β1. Utilizing specific small interfering RNA of caveolin 3, the proliferation of FS cells was inhibited. Furthermore, caveolin 3 drove activation of the MAPK signaling cascades, which resulted in upregulation of cyclin D1 in FS cells. These findings suggest that matricrine signaling in the proliferation of FS cells was transduced by a caveolin 3-mediated integrin β1 signaling pathway and subsequent activation of the MAPK pathway. |
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Authors:
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Kotaro Horiguchi; Ken Fujiwara; Cimi Ilmiawati; Motoshi Kikuchi; Takehiro Tsukada; Tom Kouki; Takashi Yashiro |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-20 |
Journal Detail:
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Title: The Journal of endocrinology Volume: - ISSN: 1479-6805 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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K Horiguchi, Anatomy, Divison of Histology and Cell Biology, Shimotsuke, 329-0498, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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