| Caveolin-1 siRNA Increases the Pulmonary Microvascular and Alveolar Epithelial Permeability in Rats. | |
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MedLine Citation:
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PMID: 20693910 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: : Increased pulmonary microvascular and epithelial permeability are important contributors to pulmonary edema in acute lung injury. In this study, we used small interfering RNA (siRNA) to knock down caveolin-1 expression in rat lungs and to confirm the important role of caveolin-1 in regulating pulmonary edema. METHOD: : After pulmonary injection of siRNA against caveolin-1 messenger RNA incorporated in liposomes with three concentrations of 0.4, 0.8, and 1.2 mg/kg, the gene silencing rate and the effects of caveolin-1 siRNA on aquaporin (AQP)-1, AQP-5, and epithelial sodium channel (ENaC) were detected. For pulmonary permeability analysis, Evans blue fluorimetry, ratios of albumin concentrations between blood and bronchoalveolar lavage, and wet/dry weight ratios were measured. The impacts of caveolin-1 suppression on interendothelial junctions were evaluated by the performance of electron microscopy and the analysis of vascular endothelial (VE)-cadherin Western blot. Alveolar wall thickness analysis and chest fluoroscopy were performed to determine the pulmonary edema degree. RESULTS: : After 72 hours of injection, the gene silencing rate of caveolin-1 siRNA is about 87%. AQP-1, AQP-5, ENaC-α, ENaC-β, ENaC-γ, and VE-cadherin protein levels were decreased by 63%, 66%, 80%, 90%, 89%, and 50%, respectively. Caveolin-1 siRNA also resulted in increasing microvascular and epithelial permeability and pulmonary edema. CONCLUSION: : These data suggest that caveolin-1 plays an important part in regulating the pulmonary permeability by modifying AQP-1, AQP-5, ENaC, and VE-cadherin. |
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Authors:
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Chengjin Gao; Rongrong Li; Jingning Huan; Wei Li |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of trauma Volume: 70 ISSN: 1529-8809 ISO Abbreviation: J Trauma Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376373 Medline TA: J Trauma Country: United States |
Other Details:
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Languages: eng Pagination: 210-9 Citation Subset: AIM; IM |
Affiliation:
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From the Department of Burn and Plastic Surgery (C.G., J.H.), Rui Jin Hospital, Shanghai Jiao Tong University, School of Medicine; Division of Radiation-Oncology (R.L.), Oral and Maxillofacial Surgery Department, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine; and Department of Burn and Plastic Surgery (W.L.), Jinshan Hospital, Fudan University, Shanghai, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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