Document Detail


Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells.
MedLine Citation:
PMID:  17498208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The P2X7 receptor has recently been described as a marker for lung alveolar epithelial type I cells. Here, we demonstrate both the expression of P2X7 protein and its partition into lipid rafts in the mouse lung alveolar epithelial cell line E10. A significant degree of colocalization was observed between P2X7 and the raft marker protein Caveolin-1; also, P2X7 protein was associated with caveolae. A marked reduction in P2X7 immunoreactivity was observed in lung sections prepared from Caveolin-1-knockout mice, indicating that Caveolin-1 expression was required for full expression of P2X7 protein. Indeed, suppression of Caveolin-1 protein expression in E10 cells using short hairpin RNAs resulted in a large reduction in P2X7 protein expression. Our data demonstrate a potential interaction between P2X7 protein and Caveolin-1 in lipid rafts, and provide a basis for further functional and biochemical studies to probe the physiologic significance of this interaction.
Authors:
K Barth; K Weinhold; A Guenther; M T Young; H Schnittler; M Kasper
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Publication Detail:
Type:  Journal Article     Date:  2007-05-11
Journal Detail:
Title:  The FEBS journal     Volume:  274     ISSN:  1742-464X     ISO Abbreviation:  FEBS J.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-01     Completed Date:  2007-09-10     Revised Date:  2010-01-14    
Medline Journal Info:
Nlm Unique ID:  101229646     Medline TA:  FEBS J     Country:  England    
Other Details:
Languages:  eng     Pagination:  3021-33     Citation Subset:  IM    
Affiliation:
Institute of Anatomy, Medical Faculty Carl Gustav Carus, Dresden University of Technology, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caveolin 1 / metabolism*
Cell Line
Cryoelectron Microscopy
Epithelial Cells / metabolism*
Lung / cytology,  metabolism
Membrane Microdomains / metabolism*,  ultrastructure
Mice
Pulmonary Alveoli / cytology,  metabolism*
Receptors, Purinergic P2 / metabolism*
Respiratory Mucosa / cytology,  metabolism*
Chemical
Reg. No./Substance:
0/Caveolin 1; 0/Receptors, Purinergic P2; 0/purinergic P2X7 receptor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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