| Caveolin-1 and doxorubicin-induced P-glycoprotein modulate plasma cholesterol membrane accessibility in erythrolymphoblastic cell line. | |
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MedLine Citation:
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PMID: 20944122 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MATERIALS AND METHODS: The present study was performed in four lymphoblastic K562 cell lines expressing none (KS), one (Cav and KR cells) or both P-gp and caveolin-1 proteins (CavKR cells). RESULTS: The CavKR cell line exhibits a significantly higher free cholesterol content than the other cell lines. Cholesterol distribution at the outer leaflet was distinct from the total cellular cholesterol by its accessibility to cholesterol oxidase (COase). When cells were ATP-deprived, cholesterol accessibility to oxidation was significantly delayed in CavKR cells. Caveolin-1 or P-gp expression did not induce detectable changes in membrane cholesterol accessibility to COase. CONCLUSION: Combination of functional P-gp, caveolae presence and lasting effect of anthracycline treatment appear determinant in free membrane cholesterol homeostasis and likely modulate cholesterol membrane order. |
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Authors:
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Miaojun Zhu; Laurence LE Moyec; Anna Starzec; Verene Stierle; Carole Marbeuf-Gueye |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anticancer research Volume: 30 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-10-14 Completed Date: 2010-11-10 Revised Date: 2011-01-11 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 3451-8 Citation Subset: IM |
Affiliation:
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Unité ANBiophy, FRE 3207, CNRS, Universités Paris 13 et Paris 6, 93017 Bobigny, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology Blotting, Western Caveolin 1 / metabolism* Cell Line, Tumor Cell Membrane / chemistry, drug effects, metabolism* Cholesterol / metabolism* Doxorubicin / pharmacology Drug Resistance, Neoplasm / physiology* Humans P-Glycoprotein / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Caveolin 1; 0/P-Glycoprotein; 23214-92-8/Doxorubicin; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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