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Cattle fetal sex determination by polymerase chain reaction using DNA isolated from maternal plasma.
MedLine Citation:
PMID:  22424578     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objective of this study was to evaluate the use of polymerase chain reaction analysis (PCR) of fetal cells/DNA in the maternal plasma of pregnant cows to determine the sex of the fetus. Plasma was harvested from 35 cows of mixed genotype at different stages of pregnancy ranging from 5 to 35 weeks. A male calf and a heifer calf provided the control samples. Fetal sex was determined by amplification of Y-specific sequences. For the 35 cows, the fetal sex predicted by this technique was in accordance with the sex of the calf at birth in 88.6% of cases. The agreement between predicted and observed fetal sex was less for cows with a gestational length of 35-48 days (63.6%). Regression analysis showed that there was a strong relationship between the probability of correctly predicting fetal sex and the stage of gestation. It was estimated that the test performed at 43.8 days post fertilization would have 95% accuracy, increasing to 99% accuracy for testing at 48.4 days and 99.9% accuracy for tests at 55.0 days or later. It was concluded that PCR analysis of fetal cells in maternal plasma can be used to predict successfully the sex of the fetus in cattle.
Authors:
A S da Cruz; D C Silva; E O A Costa; P De M-Jr; C C da Silva; D M Silva; A D da Cruz
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-27
Journal Detail:
Title:  Animal reproduction science     Volume:  -     ISSN:  1873-2232     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-3-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Pontifícia Universidade Católica de Goiás, Departamento de Biologia, Núcleo de Pesquisas Replicon, Rua 235, n. 40, Departamento de Biologia, Bloco L, Área IV Setor Universitário, Goiânia, GO, Brazil(1); Universidade Federal de Goiás, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Biologia Molecular e Celular, Campus Samambaia, Goiânia, GO, Brazil(2).
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