Document Detail


Cationic liposome coupled endostatin gene for treatment of peritoneal colon cancer.
MedLine Citation:
PMID:  20373131     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Therapy targeting cancer blood vessels requires unwavering pharmacokinetics of antiangiogenic therapeutics to neutralize the excess pro-angiogenic factors constantly secreted by tumor cells. Gene therapies have been explored to effectively create a microenvironment less favorable for angiogenesis and tumor expansion. In this study, we examined the inhibitory effect of cationic liposome coupled with the murine endostatin gene (Lipo/mEndo) on growth of intraperitoneal disseminated colon cancer models. Intraperitoneal injection of Lipo/mEndo inhibited bioluminescent signals emitted from CT26 colon cancer cells stably expressing luciferase in the living mice and prolonged their survival times. Endostatin gene therapy suppressed the colony forming capability and VEGF concentration of ascites obtained from treated mice by 74 and 60%, respectively. When tested in a similar model using HCT116 human colon cancer cells, Lipo/mEndo and bevacizumab displayed comparable repressive effects on ascites formation and tumor foci dissemination on mesentery of experimental mice. Our results implicate that cationic liposome coupled endostatin gene therapy may be a clinically effective treatment for intraperitoneal disseminated colon cancer.
Authors:
Keng-Li Lan; Fu Ou-Yang; Sang-Hue Yen; How-Ling Shih; Keng-Hsin Lan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-07
Journal Detail:
Title:  Clinical & experimental metastasis     Volume:  27     ISSN:  1573-7276     ISO Abbreviation:  Clin. Exp. Metastasis     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-31     Completed Date:  2010-06-24     Revised Date:  2010-07-08    
Medline Journal Info:
Nlm Unique ID:  8409970     Medline TA:  Clin Exp Metastasis     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  307-18     Citation Subset:  IM    
Affiliation:
Cancer Center, Taipei Veterans General Hospital, 11217, Taipei, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Base Sequence
Cations*
Cell Line, Tumor
Colonic Neoplasms / metabolism,  pathology,  therapy*
DNA Primers
Endostatins / genetics,  therapeutic use*
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Gene Therapy*
Humans
Liposomes*
Mice
Peritoneal Neoplasms / metabolism,  pathology,  secondary,  therapy*
Reverse Transcriptase Polymerase Chain Reaction
Vascular Endothelial Growth Factor A / metabolism
Chemical
Reg. No./Substance:
0/Cations; 0/DNA Primers; 0/Endostatins; 0/Liposomes; 0/Vascular Endothelial Growth Factor A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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