Document Detail


Cation and strong co-solute effects on protein kinetic stability.
MedLine Citation:
PMID:  18031274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Degradation of proteins is important for the operating life of biocatalysts and the shelf life of protein pharmaceuticals. We have previously found that the deactivating effects of salts on proteins can be correlated to an indicator of ion hydration, the B-viscosity coefficient of the anion in solution. Here, we test the influence of cations on protein kinetic stability by observing deactivation of mRFP (monomeric red fluorescent protein) in ammonium, caesium and chloride salt solutions, and we find that mRFP deactivation does not depend on cation hydration. We also measure mRFP deactivation in solutions containing denaturants (guanidinium chloride or urea) or stabilizing co-solutes (glycerol or sucrose) frequently encountered in many protein formulations to test whether hydration of these co-solutes can be used to indicate their relative effects on protein kinetic stability. We find that mRFP deactivation in solutions containing kosmotropic salts or stabilizers reaches a limiting rate and that hydration of denaturants is not an indicator of their denaturing strength.
Authors:
J M Broering; A S Bommarius
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochemical Society transactions     Volume:  35     ISSN:  0300-5127     ISO Abbreviation:  Biochem. Soc. Trans.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-22     Completed Date:  2008-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506897     Medline TA:  Biochem Soc Trans     Country:  England    
Other Details:
Languages:  eng     Pagination:  1602-5     Citation Subset:  IM    
Affiliation:
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332-0363, USA.
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MeSH Terms
Descriptor/Qualifier:
Cations / chemistry
Cesium / chemistry*
Glycerol / chemistry
Guanidine / chemistry
Kinetics
Luminescent Proteins / chemistry*
Protein Denaturation
Quaternary Ammonium Compounds / chemistry*
Salts / chemistry
Solubility
Solutions / chemistry
Sucrose / chemistry
Urea / chemistry
Chemical
Reg. No./Substance:
0/Cations; 0/Luminescent Proteins; 0/Quaternary Ammonium Compounds; 0/Salts; 0/Solutions; 0/red fluorescent protein; 113-00-8/Guanidine; 56-81-5/Glycerol; 57-13-6/Urea; 57-50-1/Sucrose; 7440-46-2/Cesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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