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Cation Modulation of Hemoglobin Interaction with Sodium n-Dodecyl Sulfate (SDS). III: Calcium Interaction with R- and Mixed Spin States of Hemoglobin S at pH 5.0: The Musical Chair Paradox.
MedLine Citation:
PMID:  23456537     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We investigate the interaction of Ca2+ (0-500 µM) and a membrane mimic (0.60 mM SDS) with both the R- and mixed spin states hemoglobin S (HbS) as a function of time. These interactions were carried out at pH 5.0. We aim at ascertaining if there is or are differences in the UV-Visible spectra of such interactions to account for the dynamics of calcium ion concentrations [Ca2+] in initiating structures which may ultimately suggest HbS polymerization and or resistance to Plasmodium attack. From our results, we conclude that (a) simultaneous interaction of 40 µM Ca2+ and 0.60 mM SDS with the R state protein would promote structural formations that can "lock up" the protein for nucleation on the membranes and or become cytotoxic to the parasite; (b) simultaneous R state HbS-SDS or R state HbS-Ca2+ would lead to enhanced hemin formation and less deoxyHb species. This condition is unlikely to precipitate polymerization in the HbS but the resulting hemin would poison the parasite; (c) the mixed spin state HbS-SDS and 40 µM Ca2+ interaction yields more toxic products to that of the interaction of the mixed spin HbS-SDS with 500 µM Ca2+ thus suggesting why the 40 µM Ca2+ is important in parasite Hb proteolysis; and (d) pronounced structural changes on interaction with SDS and Ca2+ are more in the R state to the mixed spin state.
Authors:
Charles O Nwamba; Ferdinand C Chilaka; Ali Akbar Moosavi-Movahedi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-3-3
Journal Detail:
Title:  Cell biochemistry and biophysics     Volume:  -     ISSN:  1559-0283     ISO Abbreviation:  Cell Biochem. Biophys.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-3-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9701934     Medline TA:  Cell Biochem Biophys     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Chemistry, University of Idaho, 875 Perimeter Dr. MS 2343, Moscow, ID, 83844-2343, USA, charlesquemo@yahoo.com.
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