Document Detail

Cathepsin X-deficient gastric epithelial cells in co-culture with macrophages: characterization of cytokine response and migration capability after Helicobacter pylori infection.
MedLine Citation:
PMID:  20736174     Owner:  NLM     Status:  MEDLINE    
Our previous studies have shown an association between Helicobacter pylori infection, the strong up-regulation of cathepsin X (CTSX, also called cathepsin Z/P), and the development of gastric cancer. In the present study, we analyzed primary and conventional gastric epithelial cell lines to establish an optimal in vitro mouse model system for the examination of H. pylori-induced overexpression of Ctsx in a functional way. Gastric epithelial cells were isolated from stomachs of wild-type C57BL6/N and Ctsx(-/-) mice and compared with the gastric cancer cell line CLS103. Indirect co-cultures of epithelial cells and macrophages were infected with H. pylori strain SS1 and analyzed for the expression of cathepsins, cytokines, and adhesion factors. Cellular interactions, migration capability, and adherence of H. pylori were assessed using time-lapse video microscopy and colony-forming assays. Isolated primary cells from wild-type and transgenic mice revealed qualities and expression profiles similar to those of corresponding tissue samples. Adherence of H. pylori was significantly higher in primary compared with commercially cells. Thus, induction of cathepsins, cytokines, and adhesion proteins was detected solely in primary cells and co-cultured macrophages. Microarray and migration experiments indicated that Ctsx is involved in B/T-cell proliferation/migration and adhesion of macrophages. Primary epithelial cells from stomach of Ctsx(-/-) mice represent an excellent model of H. pylori gastritis to elaborate the special functions of Ctsx in regulating the immune response to H. pylori.
Anja Bernhardt; Doerthe Kuester; Albert Roessner; Thomas Reinheckel; Sabine Krueger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-24
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  285     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-25     Completed Date:  2010-11-16     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  33691-700     Citation Subset:  IM    
Institute of Pathology, Otto-von-Guericke University, D-39120 Magdeburg, Germany.
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MeSH Terms
Cathepsin Z / metabolism*
Cell Movement
Coculture Techniques
Cytokines / metabolism*
Epithelial Cells / cytology*,  microbiology
Helicobacter Infections / metabolism*
Helicobacter pylori / metabolism*
Macrophages / cytology*,  metabolism
Mice, Inbred C57BL
Mice, Transgenic
Oligonucleotide Array Sequence Analysis
RNA, Messenger / metabolism
Stomach / metabolism*,  microbiology
Reg. No./Substance:
0/Cytokines; 0/RNA, Messenger; EC Z

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