| Cathepsin L is involved in 6- hydroxydopamine induced apoptosis of SH-SY5Y neuroblastoma cells. | |
| | |
MedLine Citation:
|
PMID: 21382360 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Our previous study reported that cathepsin L may contribute to the death of dopaminergic neurons in rodent model of Parkinson's disease (PD). In this study we detected the changes in the expression of lysosomal cathepsin L in cellular models of PD. In human neuroblastoma SH-SY5Y cells, treatment with 6-hydroxydopamine caused an increase in cathepsin L immunoreactivity in the cytoplasm and an increased production of the active form of cathepsin L. The contribution of cathepsin L to 6-OHDA-induced NF-κB activation and death of SH-SY5Y neuroblastoma cells were evaluated with an irreversible inhibitor of cathepsin L, Z-FY(t-Bu)-DMK. 6-OHDA- induced IκB-α degradation, NF-κB p65 nuclear translocation, p53 and PUMA expression were partially blocked by Z-FY(t-Bu)-DMK. In addition, Z-FY(t-Bu)- DMK modulated the Bcl-2 family levels, and suppressed caspase-3 activation. These data indicate that cathepsin L may be involved in 6-OHDA-induced apoptosis and Parkinsonian neurodegeneration. |
| | |
Authors:
|
Bei Xiang; Xifeng Fei; Wenzhuo Zhuang; Yun Fang; Zhenghong Qin; Zhongqin Liang |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-4 |
Journal Detail:
|
Title: Brain research Volume: - ISSN: 1872-6240 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-8 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0045503 Medline TA: Brain Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2010. Published by Elsevier B.V. |
Affiliation:
|
Department of Pharmacology and Laboratory of Aging and Nervous Diseases, Soochow University School of Medicine, 199 Ren Ai Road, Suzhou 215123, China; Department of Pharmacy, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Distinct neural responses to chord violations: A multiple source analysis study.
Next Document: Strategies for evaluating the environment-public health interaction of long-term latency disease: th...