Document Detail


Cathepsin B and D expression in squamous cell carcinoma.
MedLine Citation:
PMID:  8977710     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To elucidate involvement of proteinases in malignancy of keratinocytes, expression of cathepsin B, a cysteine proteinase, and cathepsin D, an aspartic proteinase, was ascertained in formalin-fixed paraffin-embedded specimens of normal skin, squamous cell carcinoma (SCC). Bowen's disease, seborrhoeic keratosis and basal cell carcinoma (BCC). Presence of procathepsin B and an intermediate form of cathepsin D was confirmed by Western blotting and enzyme activity analysis. Cathepsin B stained more intensely in SCC tumour cells than in normal epidermis; staining patterns were diffuse, granular or both. Diffuse and granular patterns (procathepsin B and mature enzyme, respectively) appeared in inner and outer parts of tumour islands, respectively. Five of 20 cases of Bowen's disease showed diffuse enhanced cathepsin B expression; 20 cases of seborrhoeic keratosis or BCC did not. Cathepsin D stained intensely in tumour cells of half the SCC cases. The staining manner and distribution of cathepsins B and D was similar in the cytoplasm of cancer cells. No enhanced staining of cathepsin D was seen in any cases of Bowen's disease, seborrhoeic keratosis, or BCC. Coexistence and localization of active mature forms of cathepsins B and D suggests that cooperation between the two enzymes may play an important part in invasion of SCC.
Authors:
A Kawada; K Hara; E Kominami; T Kobayashi; M Hiruma; A Ishibashi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The British journal of dermatology     Volume:  135     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-09     Completed Date:  1997-01-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  905-10     Citation Subset:  IM    
Affiliation:
Department of Dermatology, National Defense Medical College, Saitama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Bowen's Disease / enzymology
Carcinoma, Basal Cell / enzymology
Carcinoma, Squamous Cell / enzymology*
Cathepsin B / metabolism
Cathepsin D / metabolism
Cathepsins / metabolism*
Epidermis / enzymology
Humans
Keratosis, Seborrheic / enzymology
Neoplasm Invasiveness
Skin Neoplasms / enzymology*
Chemical
Reg. No./Substance:
EC 3.4.-/Cathepsins; EC 3.4.22.1/Cathepsin B; EC 3.4.23.5/Cathepsin D

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