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Cathelicidin LL-37 in bronchoalveolar lavage and epithelial lining fluids from COPD patients and healthy individuals.
MedLine Citation:
PMID:  23241112     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Innate immunity is currently under scope of interest concerning its role in the development of chronic obstructive pulmonary disease (COPD). Antimicrobial peptides constitute a potent part of this fast response system. Here, we focus on the role of a specific antimicrobial peptide, the only human cathelicidin, the pleiotropic LL-37 peptide, in the development of COPD under clinical conditions. A cross-sectional study was conducted in groups of 43 patients with COPD (previously classified according to GOLD) and 12 healthy individuals. Bronchoalveolar lavage fluid (BALF) sampling, followed by LL-37 measurements by mass spectrometry combined with previous immunoaffinity purification, was performed. Based on urea levels, concentrations of LL-37 in epithelial lining fluid (ELF) were calculated. Additionally, an antimicrobial assay of growth inhibition of two bacterial species, often involved in COPD development mechanisms, by purchased LL-37 was conducted. Altogether, 55 BALF samples were analyzed. LL-37 levels were significantly higher in BALF from patients in early stages of COPD (GOLD I-II) compared to BALFs from healthy individuals. The same was true for ELF. Cathelicidin’s concentration was significantly lower in both BALF and ELF from patients in advanced COPD (GOLD III-IV). The significantly elevated LL-37 levels both in BALF and ELF in patients with COPD at stage GOLD I-II together with reduced levels in advanced (COPD stage III-IV) further supports the innate immunity involvement in COPD pathology and suggests a profound change in non-specific immunity during the disease progression.
Authors:
M Golec; C Reichel; M Lemieszek; B Mackiewicz; J Buczkowski; J Sitkowska; C Skòrska; J Dutkiewicz; J Milanowski; R Ziesche
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biological regulators and homeostatic agents     Volume:  26     ISSN:  0393-974X     ISO Abbreviation:  J. Biol. Regul. Homeost. Agents     Publication Date:    2012 Oct-Dec
Date Detail:
Created Date:  2012-12-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809253     Medline TA:  J Biol Regul Homeost Agents     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  617-25     Citation Subset:  IM    
Affiliation:
Unit of Fibroproliferative Diseases, Institute of Rural Health, Lublin, Poland.
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