Document Detail

Catestatin, a chromogranin A-derived peptide, is sympathoinhibitory and attenuates sympathetic barosensitivity and the chemoreflex in rat CVLM.
MedLine Citation:
PMID:  22129620     Owner:  NLM     Status:  MEDLINE    
Hypertension is a major cause of morbidity. The neuropeptide catestatin [human chromogranin A-(352-372)] is a peptide product of the vesicular protein chromogranin A. Studies in the periphery and in vitro studies show that catestatin blocks nicotine-stimulated catecholamine release and interacts with β-adrenoceptors and histamine receptors. Catestatin immunoreactivity is present in the rostral ventrolateral medulla (RVLM), a key site for blood pressure control in the brain stem. Recently, we reported that microinjection of catestatin into the RVLM is sympathoexcitatory and increases barosensitivity. Here, we report the effects of microinjection of catestatin (1 mM, 50 nl) into the caudal ventrolateral medulla (CVLM) in urethane-anesthetized, bilaterally vagotomized, artificially ventilated Sprague-Dawley rats (n = 8). We recorded resting arterial pressure, splanchnic sympathetic nerve activity, phrenic nerve activity, heart rate, and measured cardiovascular homeostatic reflexes. Homeostatic reflexes were evaluated by measuring cardiovascular responses to carotid baroreceptor and peripheral chemoreceptor activation. Catestatin decreased basal levels of arterial pressure (-23 ± 4 mmHg), sympathetic nerve activity (-26.6 ± 5.7%), heart rate (-19 ± 5 bpm), and phrenic nerve amplitude (-16.8 ± 3.3%). Catestatin caused a 15% decrease in phrenic inspiratory period (T(i)) and a 16% increase in phrenic expiratory period (T(e)) but had no net effect on the phrenic interburst interval (T(tot)). Catestatin decreased sympathetic barosensitivity by 63.6% and attenuated the peripheral chemoreflex (sympathetic nerve response to brief hypoxia; range decreased 39.9%; slope decreased 30.1%). The results suggest that catestatin plays an important role in central cardiorespiratory control.
Andrea H Gaede; Paul M Pilowsky
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-30
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  302     ISSN:  1522-1490     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-13     Completed Date:  2012-04-19     Revised Date:  2012-05-23    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R365-72     Citation Subset:  IM    
Australian School of Advanced Medicine, Macquarie University, North Ryde, NSW, Australia.
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MeSH Terms
Anoxia / physiopathology
Baroreflex / drug effects*,  physiology
Blood Pressure / drug effects,  physiology
Chemoreceptor Cells / cytology,  drug effects*,  physiology
Chromogranin A / administration & dosage,  metabolism*,  pharmacology
Heart Rate / drug effects,  physiology
Medulla Oblongata / cytology,  drug effects*,  physiology
Models, Animal
Peptide Fragments / administration & dosage,  metabolism,  pharmacology*
Phrenic Nerve / drug effects,  physiology
Rats, Sprague-Dawley
Sympathetic Nervous System / drug effects*,  physiology
Sympatholytics / administration & dosage,  pharmacology*
Reg. No./Substance:
0/Chromogranin A; 0/Peptide Fragments; 0/Sympatholytics; 0/chromogranin A (344-364)

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