| Catestatin, a chromogranin A-derived peptide, is sympathoinhibitory and attenuates sympathetic barosensitivity and the chemoreflex in rat CVLM. | |
| | |
MedLine Citation:
|
PMID: 22129620 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Hypertension is a major cause of morbidity. The neuropeptide catestatin [human chromogranin A-(352-372)] is a peptide product of the vesicular protein chromogranin A. Studies in the periphery and in vitro studies show that catestatin blocks nicotine-stimulated catecholamine release and interacts with β-adrenoceptors and histamine receptors. Catestatin immunoreactivity is present in the rostral ventrolateral medulla (RVLM), a key site for blood pressure control in the brain stem. Recently, we reported that microinjection of catestatin into the RVLM is sympathoexcitatory and increases barosensitivity. Here, we report the effects of microinjection of catestatin (1 mM, 50 nl) into the caudal ventrolateral medulla (CVLM) in urethane-anesthetized, bilaterally vagotomized, artificially ventilated Sprague-Dawley rats (n = 8). We recorded resting arterial pressure, splanchnic sympathetic nerve activity, phrenic nerve activity, heart rate, and measured cardiovascular homeostatic reflexes. Homeostatic reflexes were evaluated by measuring cardiovascular responses to carotid baroreceptor and peripheral chemoreceptor activation. Catestatin decreased basal levels of arterial pressure (-23 ± 4 mmHg), sympathetic nerve activity (-26.6 ± 5.7%), heart rate (-19 ± 5 bpm), and phrenic nerve amplitude (-16.8 ± 3.3%). Catestatin caused a 15% decrease in phrenic inspiratory period (T(i)) and a 16% increase in phrenic expiratory period (T(e)) but had no net effect on the phrenic interburst interval (T(tot)). Catestatin decreased sympathetic barosensitivity by 63.6% and attenuated the peripheral chemoreflex (sympathetic nerve response to brief hypoxia; range decreased 39.9%; slope decreased 30.1%). The results suggest that catestatin plays an important role in central cardiorespiratory control. |
| | |
Authors:
|
Andrea H Gaede; Paul M Pilowsky |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-11-30 |
Journal Detail:
|
Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 302 ISSN: 1522-1490 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-02-13 Completed Date: 2012-04-19 Revised Date: 2012-05-23 |
Medline Journal Info:
|
Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: R365-72 Citation Subset: IM |
Affiliation:
|
Australian School of Advanced Medicine, Macquarie University, North Ryde, NSW, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anoxia / physiopathology Baroreflex / drug effects*, physiology Blood Pressure / drug effects, physiology Chemoreceptor Cells / cytology, drug effects*, physiology Chromogranin A / administration & dosage, metabolism*, pharmacology Heart Rate / drug effects, physiology Male Medulla Oblongata / cytology, drug effects*, physiology Microinjections Models, Animal Peptide Fragments / administration & dosage, metabolism, pharmacology* Phrenic Nerve / drug effects, physiology Rats Rats, Sprague-Dawley Sympathetic Nervous System / drug effects*, physiology Sympatholytics / administration & dosage, pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Chromogranin A; 0/Peptide Fragments; 0/Sympatholytics; 0/chromogranin A (344-364) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Dramatic increase of nitrite levels in hearts of anoxia-exposed crucian carp supporting a role in ca...
Next Document: Supramolecular double helix from capped ?-peptide.