Document Detail


The Wnt/β-catenin signaling pathway tips the balance between apoptosis and reprograming of cell fusion hybrids.
MedLine Citation:
PMID:  20827748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-cell fusion contributes to cell differentiation and developmental processes. We have previously showed that activation of Wnt/β-catenin enhances somatic cell reprograming after polyethylene glycol (PEG)-mediated fusion. Here, we show that neural stem cells and ESCs can fuse spontaneously in cocultures, although with very low efficiency (about 2%), as the hybrids undergo apoptosis. In contrast, when Wnt/β-catenin signaling is activated in ESCs and leads to accumulation of low amounts of β-catenin in the nucleus, activated ESCs can reprogram somatic cells with very high efficiency after spontaneous fusion. Furthermore, we also show that different levels of β-catenin accumulation in the ESC nuclei can modulate cell proliferation, although in our experimental setting, cell proliferation does not modulate the reprograming efficiency per se. Overall, the present study provides evidence that spontaneous fusion occurs, while the survival of the reprogramed clones is strictly dependent on induction of a Wnt-mediated reprograming pathway.
Authors:
Frederic Lluis; Elisa Pedone; Stefano Pepe; Maria Pia Cosma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  28     ISSN:  1549-4918     ISO Abbreviation:  Stem Cells     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-22     Completed Date:  2011-03-15     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1940-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 AlphaMed Press.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / genetics,  physiology*
Blotting, Western
Cell Differentiation / genetics,  physiology
Cell Fusion / methods*
Cell Proliferation
Cell Survival / genetics,  physiology
Cells, Cultured
Embryonic Stem Cells / cytology,  metabolism
Flow Cytometry
Mice
Neural Stem Cells / cytology*,  metabolism*
Nuclear Reprogramming / genetics,  physiology
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics,  physiology*
Wnt Proteins / metabolism*
beta Catenin / metabolism*
Grant Support
ID/Acronym/Agency:
242630//European Research Council; TGM06C02//Telethon
Chemical
Reg. No./Substance:
0/Wnt Proteins; 0/beta Catenin
Comments/Corrections

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